FastQCFastQC Report
Fri 1 Jul 2022
EGAF00005499151

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005499151
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences38888
Sequences flagged as poor quality0
Sequence length200
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTCGAGCCTCAACGTCAGTTACCGTCCAGTAAGCCGCCTTCGCCACTGGT1499438.556881300144No Hit
TTCGAGCCTCAGCGTCAGTTACAGTCCAGAAAGGCGCCTTCGCCACTGGT599815.423781114996915No Hit
TTCGAGCCTCAACGTCAGTCATCGTCCAGAAAGCCGCCTTCGCCACTGGT500912.880580127545773No Hit
TTCGCGCCTCAGCGTCAGTTTTCGTCCAGAAAGTCGCCTTCGCCACTGGT23856.132997325653157No Hit
TTCGCACCTGAGCGTCAGTCTTTGTCCAGGGGGCCGCCTTCGCCACCGGT20795.346122197078791No Hit
TTCGAGCCTCAGCGTCAGTTACAGTCCAGAAAGTCGCCTTCGCCACTGGT15764.052664060892821No Hit
TTCGTGCCTCAGCGTCAGTTACAGTCCAGAGAGCCGCCTTCGCAACTGGT7922.0366179798395394No Hit
TTCGAGCCTCAGTGTCAGTTACAGTCCAGAAAGGCGCCTTCGCCACTGGT6661.712610573955976No Hit
TTCGCACCTGAGCGTCAGTCTTCGTCCAGGGGGCCGCCTTCGCCACCGGT4751.221456490434067No Hit
TTCGCGCCTCAGCGTCAGTTACTGTCCAGCAATCCGCCTTCGCCACTGGT4301.105739559761366No Hit
TTCGGGACTGAGCGTCAGTTGCAGGCCAGATCGTCGCCTTCGCCACTGGT3120.8023040526640608No Hit
TTCGCTCCTCAGCGTCAGTAACGGCCCAGAGACCTGCCTTCGCCATTGGT2720.699444558732771No Hit
TTCGCCCCTGAGCGTCAGTCTTTGTCCAGGGGGCCGCCTTCGCCACCGGT2140.5502982925324007No Hit
TTCGAGCCTCAGCGTCAGTTACAGTCCAGAGAGTCGCCTTCGCCACTGGT1660.4268668998148529No Hit
TTCGCACCTTAGCGTCAGTCTTTGTCCAGGGGGCCGCCTTCGCCACCGGT1470.37800864019749025No Hit
TTCGTGCCTCAGCGTCAGTTACAGTCCAGAGAGCCGCCTTCGCTACTGGT1460.375437152849208No Hit
TCGAGCCTCAACGTCAGTTACCGTCCAGTAAGCCGCCTTCGCCACTGGTG1110.28543509565932934No Hit
TTCGAGCCTCAGCGTCAGTTACCGTCCAGTAAGCCGCCTTCGCCACTGGT1100.2828636083110471No Hit
TTCGAGCCTCAGCGTCAGTTACAGTCCAGAAAGCCGCCTTCGCCACTGGT680.17486113968319275No Hit
TTCGTGCCTCAGTGTCAGTTACAGACCAGGCGACCGCCTTCGCCACTGGT620.1594322155934993No Hit
TTCGCGCCTCAGCGTCAGTTTTCGTCCAGAAAGCCGCCTTCGCCACTGGT520.13371734211067682No Hit
TTCGCCCCTGAGCGTCAGTCTTCGTCCAGGGGGCCGCCTTCGCCACCGGT520.13371734211067682No Hit
TTCGAGCCTCAACGTCAGTCATCGTCCAGAAAGTCGCCTTCGCCACTGGT510.13114585476239457No Hit
TTCGTGCCTCAGCGTCAGTTACAGTCCAGAGAGCCGCCTTCGCCACTGGT500.12857436741411232No Hit
TTCGAGCCTCAACGTCAGTCATCGTCCAGTAAGCCGCCTTCGCCACTGGT460.11828841802098333No Hit
TTCGAGCCTCAACGTCAGTTACAGTCCAGAAAGGCGCCTTCGCCACTGGT440.11314544332441884No Hit
TTCGAGCCTCAACGTCAGTTACCGTCCAGAAAGCCGCCTTCGCCACTGGT440.11314544332441884No Hit
TTCGCACCTTAGCGTCAGTCTTCGTCCAGGGGGCCGCCTTCGCCACCGGT430.1105739559761366No Hit
TTCGCCCCTTAGCGTCAGTCTTTGTCCAGGGGGCCGCCTTCGCCACCGGT420.10800246862785436No Hit
TTCGAGCCTCAGCGTCAGTTACAGACCAGAGAGCCGCCTTCGCCACTGGT420.10800246862785436No Hit
TTCGCACCTGAGCGTCAGTCTTTGTCCAGGGGGCCGCCTTCGCCACTGGT400.10285949393128986No Hit
TTCGAGCCTCAGCGTCAGTTACAGTCCAGTAAGCCGCCTTCGCCACTGGT400.10285949393128986No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)