FastQCFastQC Report
Thu 12 May 2022
EGAF00005555582

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005555582
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences46672178
Sequences flagged as poor quality0
Sequence length101
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AATGATACGGCGACCACCGAGATCGGAAGAGCACACGTCTGAACTCCAGT1218400.26105488370394886Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
GTCAGTGAAAAAACGATCAGAGTAGTGGTATTTCACCGGCGGCCCGCAGG956160.20486723375112256No Hit
CCCGCCTCACCGGGTCAGTGAAAAAACGATCAGAGTAGTGGTATTTCACC845490.18115503416189405No Hit
GAAAAAACGATCAGAGTAGTGGTATTTCACCGGCGGCCCGCAGGGCCGGC839780.17993160722004448No Hit
GTGAAAAAACGATCAGAGTAGTGGTATTTCACCGGCGGCCCGCAGGGCCG839530.17987804211751163No Hit
GGACGGACGGACGGACGGACGGGGCCCCCCGAGCCACCTTCCCCGCCGGG614030.1315623196329085No Hit
CCCCGCCTCACCGGGTCAGTGAAAAAACGATCAGAGTAGTGGTATTTCAC478690.10256431572574136No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)