FastQCFastQC Report
Thu 12 May 2022
EGAF00005555586

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005555586
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences54674365
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AATGATACGGCGACCACCGAGATCGGAAGAGCACACGTCTGAACTCCAGT1046200.19135110211156545Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
CCCGCCTCACCGGGTCAGTGAAAAAACGATCAGAGTAGTGGTATTTCACC707540.12940982487862457No Hit
GTCAGTGAAAAAACGATCAGAGTAGTGGTATTTCACCGGCGGCCCGCAGG617680.11297433449844366No Hit
GAAAAAACGATCAGAGTAGTGGTATTTCACCGGCGGCCCGCAGGGCCGGC550440.10067606637955465No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)