FastQCFastQC Report
Thu 12 May 2022
EGAF00005555598

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005555598
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences50518758
Sequences flagged as poor quality0
Sequence length101
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AATGATACGGCGACCACCGAGATCGGAAGAGCACACGTCTGAACTCCAGT7037191.392985552020103Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACTAAGACATCTCGTAT1299610.2572529593858978TruSeq Adapter, Index 25 (97% over 38bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTAAGACATCTCGTA1038520.2055711662586796TruSeq Adapter, Index 25 (97% over 38bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACTAAGACATCGCGTAT793290.15702880106434922TruSeq Adapter, Index 25 (97% over 38bp)
GTCAGTGAAAAAACGATCAGAGTAGTGGTATTTCACCGGCGGCCCGCAGG623320.12338387258055711No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTAAGACATCGCGTA585560.11590942120944463TruSeq Adapter, Index 25 (97% over 38bp)
GAAAAAACGATCAGAGTAGTGGTATTTCACCGGCGGCCCGCAGGGCCGGC584830.11576492042817046No Hit
CCCGCCTCACCGGGTCAGTGAAAAAACGATCAGAGTAGTGGTATTTCACC572860.11339550350782575No Hit
GTGAAAAAACGATCAGAGTAGTGGTATTTCACCGGCGGCCCGCAGGGCCG546250.1081281531109692No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)