FastQCFastQC Report
Sat 4 Jun 2022
EGAF00005555599

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005555599
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences50518758
Sequences flagged as poor quality0
Sequence length101
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGTGGTCGCCGTATCATTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGT2617770.5181778221863649Illumina Single End PCR Primer 1 (100% over 31bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTCGAATGAGTGTAGATC1506610.2982278384595282Illumina Single End PCR Primer 1 (96% over 33bp)
CGGTGGTCGCCGTATCATTAGATCGGAAGAGCGTCGTGGAGGGAAAGAGT559150.11068166006773167Illumina Single End PCR Primer 1 (96% over 31bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)