FastQCFastQC Report
Thu 12 May 2022
EGAF00005555612

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005555612
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences51355283
Sequences flagged as poor quality0
Sequence length101
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AATGATACGGCGACCACCGAGATCGGAAGAGCACACGTCTGAACTCCAGT1859630.36211074915116326Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
GTCAGTGAAAAAACGATCAGAGTAGTGGTATTTCACCGGCGGCCCGCAGG733020.14273507167704635No Hit
CCCGCCTCACCGGGTCAGTGAAAAAACGATCAGAGTAGTGGTATTTCACC635420.12373021096972632No Hit
GTGAAAAAACGATCAGAGTAGTGGTATTTCACCGGCGGCCCGCAGGGCCG616800.1201044885683913No Hit
GAAAAAACGATCAGAGTAGTGGTATTTCACCGGCGGCCCGCAGGGCCGGC530000.10320262474261899No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)