FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005560760

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005560760
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8240098
Sequences flagged as poor quality0
Sequence length35-76
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGTACG950.007504903718.29534746
CGTCGGA2352.8850573E-614.83848857
ATCGTCG3052.0657353E-713.71139955
TCGTCGG2701.2162194E-512.91103856
GATCGTC5109.684481E-79.56340554
AGTTCGC3150.0084290728.806128
GTCGGAC4500.00208178267.751731458
TCGGACT6000.0047057626.3976459
CAGTTCG6100.005976516.252425727
GCCTACA23950.06.079624722
TTCAGCG11250.00498938475.79607370
GTGCCTA25950.05.610889420
TCGATCA7500.00757863565.581246458
TGCCTAC28650.05.56622721
CGAATGT7550.0086303575.5157891
CCTCGAT9600.00381866595.08372156
GTCGCCA10350.00173812065.05940160
Produced by FastQC (version 0.11.5)