FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005560788

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005560788
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7189462
Sequences flagged as poor quality0
Sequence length35-76
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGTACG1253.8235157E-927.81860246
TCGTCGG3600.020.33903156
CGTCGGA3550.019.65006457
GTCGGAC4450.016.46602858
ATCGTCG4450.015.66591955
GATCGTC5350.015.632754
AGTTCGC3959.094947E-1214.92156228
GTTCGCA2952.2435324E-612.93075129
TCGGACT6200.011.82307659
TAGATCG3003.616984E-511.60922752
CGCATTA3551.759781E-510.74833932
CAGTTCG5653.601599E-910.43152427
ACGGTAC3501.7620395E-49.93251145
GCCTACA22750.09.14251822
TTCGCAT4201.1163774E-49.08351430
CGGACTG9001.6370905E-118.92485560
GTGCCTA21600.08.82658820
TACGAGA4108.503675E-48.48702449
TGCCTAC25800.08.33038121
TCGCATT5351.7663496E-47.779849531
Produced by FastQC (version 0.11.5)