FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005560802

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005560802
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6815229
Sequences flagged as poor quality0
Sequence length35-76
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGTCGC4801.8189894E-1214.53059169
ATCGTCG4607.2759576E-1213.63131355
TCGTCGG4354.1836756E-1113.61691656
CGTCGGA4456.184564E-1113.31395957
GTCGGAC5351.1641532E-1011.67563634
GGTCGCC8953.092282E-1111.51140570
GTGCCTA21950.011.21814520
GATCGTC5956.002665E-1111.1211854
GCCTACA22550.011.07361922
GCGCTAT2550.001442706410.90141243
GTGGTCG7301.8189894E-1210.82345268
AAGCGCG2350.00963716710.36952552
CGTGTAG5855.8989826E-910.12315955
TGCCTAC26600.010.03951521
TGAACGT8051.5716068E-98.64721851
ATTGATG28100.08.63963413
TCGGACT6909.2022674E-88.55085435
GGATCGT7757.723429E-98.5049229
TTGATGG34300.07.98797314
ATGGTGC33300.07.915285617
Produced by FastQC (version 0.11.5)