FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005560838

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005560838
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9482497
Sequences flagged as poor quality0
Sequence length35-76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCTGA17300.06.60986967
CGGGTCG13005.0986273E-96.472671557
AACGGCG11104.8974107E-76.33206159
TCGAAGT8401.2031157E-46.16663557
ACGTCTG18757.2759576E-126.06318966
CGCCACG15454.632966E-95.93729162
ACGGCGA11951.7901748E-65.88966360
GTCGCCA15808.174538E-95.79088160
GGGTCGC16602.6384441E-85.49814658
GGTCGCC16652.7526767E-85.48778659
CGGCGAG15204.131438E-65.099290461
TAACGGC13206.08374E-55.052783558
Produced by FastQC (version 0.11.5)