FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005560879

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005560879
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences799819
Sequences flagged as poor quality0
Sequence length35-76
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG12320.15403485038489959No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCATGCG200.006648617353.0941456
TCGTACC857.094059E-1140.3218461
GCGAACT1300.034.27142320
TGCGAAC1004.0927262E-1034.2671319
ATGCGAA1056.91216E-1032.6353718
CGTACCG1251.3460522E-1030.1531932
GTACCGT1302.1282176E-1028.9934563
AATGCGA1202.8849172E-928.55416117
TAATGCG1254.461981E-927.41199316
CGAACTG1653.6379788E-1227.00341621
CGTGAGT1501.1277734E-925.127667
TACCGTG1653.3978722E-922.8433284
TAGGCAC4750.020.936728
ACCGTGA1851.2711098E-820.3737815
GTAGGCA5600.018.37003327
TGTAGGC5400.017.13892626
ACTGTAG5750.016.68973724
GCACCAT6850.015.556431
CATCAAT6650.015.5395235
CCGTGAG2453.149362E-715.3842826
Produced by FastQC (version 0.11.5)