FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005560890

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005560890
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences871482
Sequences flagged as poor quality0
Sequence length35-76
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG8720.10005943897865935No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGCGAT200.00774871551.07792323
GGCGGAC750.00215355322.6947821
CGTTTCC1450.005274418314.25087639
CCCGTCT2500.001452753110.89036816
AAGATTC3750.00280943238.580557
CTTCCAT5101.2516187E-48.0122221
AGTTAAT3950.0069577197.75867181
CTTTCAT6706.207716E-67.62357041
CTCAGAA8201.9404542E-67.059551
CTCAAAC6750.00306556446.05367951
CTTTTAA8800.0017159125.4173551
CAAAAAT12504.0630293E-55.17589571
Produced by FastQC (version 0.11.5)