FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005560908

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005560908
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8245893
Sequences flagged as poor quality0
Sequence length35-76
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCGGA3950.017.64624857
TCGTCGG4500.015.48476556
ATCGTCG4550.015.31040455
GTCGGAC5700.012.84403858
TCGGACT7101.8189894E-1210.80631759
GATCGTC6502.7284841E-1110.71447754
CGTATTG4850.00728966068.96340870
CGGCTAG3905.1442604E-48.92655753
GTGCCTA25550.08.41512320
CGGACTG9801.03682396E-108.18747960
GCCTACA28350.07.9511622
CGCGATC5351.7634613E-47.7809863
TGCCTAC32800.06.872378321
CTACAGT36000.06.839692624
ATTGATG33700.06.58581813
TACAGTT40800.06.290138225
TTGATGG37950.06.031034514
CCTACAG42350.05.97784823
AGACCGG7350.00623098065.681081351
TGAACGA7600.0089626165.496879653