FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005561036

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005561036
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences33337
Sequences flagged as poor quality0
Sequence length219
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTGTTTGATCCCCATGCTTTCGTGCCTCAGCGTCAGTATATGGCCAGAT738822.161562228154903No Hit
CCTGTTTGATACCCACACTTTCGAGCCTCAATGTCAGTTGCAGCTTAGCA516715.49929507754147No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTTACAGTCCAGTA22956.884242733299336No Hit
CCTGTTTGATACCCACACTTTCGAGCATCAGCGTCAGTTACAGTCCAGTA19565.8673545909949905No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTTACCGTCCAGTA14744.421513633500315No Hit
CCTGTTTGCTACCCACACTTTCGAGCCTCAGCGTCAGTTGGTGCCCAGTA12073.620601733809281No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAACGTCAGATATAGTTTGGTA10393.1166571677115518No Hit
CCTGTTTGATACCCACACTTTCGAGCATCAGTGTCAGTTGCAGTCTAGTG10133.0386657467678555No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTAAAAGCCCAGCA9252.774694783573807No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTAATCGTCCAGTA8212.4627290997990223No Hit
CCTGTTTGATACCCACACTTTCGAGCATCAGTGTCAGTTGCAGTCCAGTG7162.1477637459879415No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTATCGTCCAGTA6862.0577736448990613No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGTGTCAGTTACAGTCCAGCA4961.4878363380028197No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAGTGTCAGTAGCAGTCCAGTA4461.3378528361880193No Hit
CCTGTTTGATACCCACACTTTCGAGCATCAGCGTCAGTTACAGTCCAGCA4391.3168551459339473No Hit
CCTGTTTGCTACCCATGCTTTCGAGCCTCAGCGTCAGTTGGTGCCCAGTA3741.1218765935747066No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTTTTCGTCCAGAA3421.0258871524132347No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCATGAGCGTCAGTCATGTCCCAGAG3180.9538950715421303No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTACCGTCCAGTA2690.806911239763626No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTTATCGTCCAGTT2630.78891321954585No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTTACTGTCCAGTA2270.6809250982391937No Hit
CCTGTTCGATACCCACGCTTTCGTGCTTCAGCGTCAGTTATAGCCCAGTA2200.6599274079851216No Hit
CCTGTTTGCTCCCCACACTTTCGCGCCTCACCGTCAGTTATCGTCCAGCA1830.5489396166421694No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAGTGTCAGTTACAGTCCAGTA1820.5459399466058733No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGTGTCAGTTACAGTCCAGTG1620.4859465458799532No Hit
CCTGTTTGATCCCCACGCTTTCGTGCTTCAGTGTCAGTTATGGTTTAGTA1530.4589495155532891No Hit
CCTGTTTGCTCCCCACACTTTCGAGCCTCAGCGTCAGTTAAAGCCCAGTT1370.410954794972553No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAGTGTCAGTAACAGTCCAGCA1300.38995710471848094No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTCATCGTCCAGTA1280.383957764645889No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCACCGTCAGTTGTCGTCCAGCA1210.3629600743918169No Hit
CCCATTCGCTCCCCCGGCTTTCGTTCCTCAGCGTCAATAACGGCCCAGTA1030.30896601373848875No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTTATCGTCCAGTA1000.2999670036296007No Hit
CCTGTTTGATACCCACACTTTCGAGCATCAGCGTCAGTTACACTCCAGTG940.2819689834118247No Hit
CCTGTTTGCTACCCACGCTTTCGTGCCTCAGCGTCAGTTAAAGCCCAGCA860.2579716231214566No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTTGTCGTCCAGCA730.21897591264960856No Hit
CTGTTTGATCCCCATGCTTTCGTGCCTCAGCGTCAGTATATGGCCAGATA730.21897591264960856No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGTGTCAGTTACAGTCCAGTA690.2069772325044245No Hit
CCTGTTCGCTACCCACGCTCTCGCGCATCAGCGTCAGAACGAGCCTGGGA670.20097789243183248No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCACCGTCAGTTACCGTCCAGTA660.1979782223955365No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTTGCCGTCCAGTA660.1979782223955365No Hit
CCTGTTTGCTACCCACGCTTTCGTGCATCAGCGTCAATTCTAGACCAGTA650.19497855235924047No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTCATCGTCCAGTA640.1919788823229445No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTTAATGTCCAGCA570.1709811920688724No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTAAAAGCCCAGTA570.1709811920688724No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTGGTGCCCAGTA560.16798152203257644No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTGAACGTCAGTTACTGTCCAGTA550.1649818519962804No Hit
CCTGTTTGCTCCCCACACTTTCGCGCCTCAGCGTCAGTTGTCGTCCAGAA510.1529831718510964No Hit
CCTGTTTGATCCCCATGCTTTCGTGCCTCAGCGTCAGTATATGTCCAGAT500.14998350181480036No Hit
CCTGTTTGCTCCCCACGCTTTCGCACATGAGCGTCAGTACATTCCCAAGG480.14398416174220835No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAACGTCAGCTATAGTTTGGTA480.14398416174220835No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAGTGTCAGTTTCAGTCCAGTA450.13498515163332034No Hit
CCTGTTCGATACCCACGCTTTCGTGCTTCAGCGTCTGTTTCACCCCAGTA430.1289858115607283No Hit
CCCTTTCGCTCCCCTGGCCTTCGTGCCTCAGCGTCAGTTAATGTCCAGGA420.12598614152443233No Hit
CTGTTTGATACCCACACTTTCGAGCCTCAATGTCAGTTGCAGCTTAGCAG390.11698713141554429No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAGCGTCAGTTGCAGTCCAGAA380.11398746137924828No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAACGTCAGATATAGTTTTGTA350.10498845127036024No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGTGTCAGTCACAGTCCAGTG340.10198878123406425No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTTCAGTCCAGAA340.10198878123406425No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCTACC1550.0213.000027
TTTGCTA1550.0213.000025
TTGCTAC1550.0213.000026
CGATACC253.6488927E-9213.07
TCGATAC253.6488927E-9213.06
CCTGTTC500.0213.01
TTCGATA253.6488927E-9213.05
TGTTCGC253.6488927E-9213.03
TGTTCGA253.6488927E-9213.03
TCCTGTT100.0022346897213.01
CTGTTCG500.0213.02
GTTCGCT253.6488927E-9213.04
GTTCGAT253.6488927E-9213.04
CTCCCCC152.6326488E-5212.999989
TCGCTAC152.6326488E-5212.999986
CCTGTTT32150.0212.337461
GATACCC10250.0209.882938
ATACCCA10250.0209.882939
TGATACC10050.0209.820897
CTGTTTG32600.0209.079762
Produced by FastQC (version 0.11.5)