FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005561145

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005561145
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences47822
Sequences flagged as poor quality0
Sequence length220
%GC52

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TACGTATGGTGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGAGCGCA1204325.182970181088205No Hit
AACGTAGGTCACAAGCGTTGTCCGGAATTACTGGGTGTAAAGGGAGCGCA937019.593492534816612No Hit
TACGTAGGGGGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGAGCGTA826417.280749445861737No Hit
TACGGAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTA28786.01815064196395No Hit
TACGTATGGAGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGAGTGTA20014.1842666555141985No Hit
TACGTATGGTGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGAGCGTA12362.5845845008573463No Hit
TACGTAGGGGGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGAGCGCA11852.477939023880223No Hit
TACGTAGGGGGCAAGCGTTATCCGGAATTACTGGGTGTAAAGGGTGCGTA9822.0534482037555937No Hit
TACGTAGGTGGCGAGCGTTATCCGGAATTATTGGGCGTAAAGAGGGAGCA9321.9488938145623353No Hit
TACGTAGGGTGCAAGCGTTATCCGGAATTATTGGGCGTAAAGGGCTCGTA8771.8338839864497511No Hit
TACGTAGGGGGCTAGCGTTATCCGGAATTACTGGGCGTAAAGGGTGCGTA4080.853163815816988No Hit
TACGTAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGTGCGCA3940.8238885868428757No Hit
TACGTAGGTGACAAGCGTTGTCCGGATTTACTGGGTGTAAAGGGCGCGTA3160.6607837397013927No Hit
TACGTATGGAGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGTGCGTA2970.6210530718079545No Hit
TACGTATGGAGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGAGCGCA2600.5436828238049433No Hit
TACGTATGGGGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGAGCGTA2250.4704947513696625No Hit
TACGTATGGTGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGAGTGTA2230.46631257580193214No Hit
TACGTAGGGGGCGAGCGTTATCCGGATTCATTGGGCGTAAAGCGCGCGTA2070.4328551712600895No Hit
TACGTAGGGAGCGAGCGTTGTCCGGAATTACTGGGTGTAAAGGGAGCGTA1700.35548492325707837No Hit
TACGTAGGTCACAAGCGTTGTCCGGAATTACTGGGTGTAAAGGGAGCGCA1540.32202751871523566No Hit
TACGTAGGGGGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGAGTGTA1460.30529881644431434No Hit
TACGTATGGGGCGAGCGTTATCCGGATTCATTGGGCGTAAAGCGCGCGTA1430.29902555309271883No Hit
TACGTATGGAGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGAGCGTA1330.2781146752540672No Hit
TACGTAGGGAGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGCGCGCA1280.2676592363347413No Hit
AACGTAGGGTGCAAGCGTTGTCCGGAATTACTGGGTGTAAAGGGAGCGCA1170.2446572707122245No Hit
AACGTAGGTCACAAGCGTTGTCCGGAATTACTGGGTGTAAAGGGAGCGTA1090.22792856844130316No Hit
TACGTAGGGGGCGAGCGTTATCCGGATTTACTGGGTGTAAAGGGAGCGTA970.20283551503492117No Hit
TACGTAGGGGGCGAGCGTTATCCGGAATCATTGGGCGTAAAGCGCGCGCA920.19238007611559535No Hit
TACGTAGGGGGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGTGCGTA760.15892267157375267No Hit
TACGTATGGTGCAAGCGTTGTCCGGAATTACTGGGTGTAAAGGGAGCGCA640.13382961816737066No Hit
TACGTATGGTGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGTGCGTA610.12755635481577515No Hit
TACGTAGGGGGCAAGCGTTATCCGGAATTACTGGGTGTAAAGGGAGCGTA590.12337417924804482No Hit
ACGTATGGTGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGAGCGCAG530.11082765254485383No Hit
TACGTATGGTGCAAGCGTTATCCGGAATTACTGGGTGTAAAGGGAGCGCA520.10873656476098865No Hit
TACGTAGGGGGCAAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTA500.10455438919325832No Hit
AACGTAGGTCACAAGCGTTATCCGGATTTACTGGGTGTAAAGGGAGCGCA480.10037221362552799No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGATCC2750.0214.000026
ACGTATG18750.0214.000022
TACGTAT18750.0214.000021
GATCCGA2750.0214.000028
CGTATGG18750.0214.000023
ATCCGAG2750.0214.000029
ATGGGGC350.0214.06
GTATGGT15150.0214.04
GTATGGA3200.0214.04
GGTGACA400.0214.07
GAGCGAG203.0352385E-7214.09
GTCACAA9600.0214.08
ATGGTGC15100.0214.06
TGACAAG400.0214.09
GGTCACA9600.0214.07
TGGAGCA3250.0214.07
AGGTGAC400.0214.06
CCGTAGG152.5909585E-5214.02
TGGGGCG152.5909585E-5214.07
GTGACAA400.0214.08
Produced by FastQC (version 0.11.5)