FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005561163

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005561163
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences36507
Sequences flagged as poor quality0
Sequence length220
%GC52

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TACGTATGGTGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGAGCGCA740120.272824389843045No Hit
TACGGAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTA650817.82671816363985No Hit
TACGTAGGGGGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGAGCGTA596216.331114580765334No Hit
AACGTAGGTCACAAGCGTTGTCCGGAATTACTGGGTGTAAAGGGAGCGCA506213.865833949653492No Hit
TACGTAGGGTGCAAGCGTTATCCGGAATTATTGGGCGTAAAGGGCTCGTA13143.599309721423289No Hit
TACGTATGGTGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGAGCGTA9962.728243898430438No Hit
TACGTAGGGGGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGAGCGCA9762.6734598844057307No Hit
TACGTAGGGAGCGAGCGTTGTCCGGAATTACTGGGTGTAAAGGGAGCGTA6321.7311748431807599No Hit
TACGTATGGAGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGAGTGTA4681.2819459281781576No Hit
TACGTAGGTGGCGAGCGTTATCCGGAATTATTGGGCGTAAAGAGGGAGCA2580.7067137809187279No Hit
TACGTAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGTGCGCA2230.6108417563754897No Hit
TACGTAGGTGACAAGCGTTGTCCGGATTTACTGGGTGTAAAGGGCGCGTA1700.46566411921001455No Hit
TACGGAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGTGCGTA1690.46292491850877915No Hit
TACGTAGGTGGCAAGCGTTGTCCGGAATTATTGGGCGTAAAGCGCGCGCA1330.36431369326430546No Hit
AACGTAGGTCACAAGCGTTGTCCGGAATTACTGGGTGTAAAGGGAGCGTA1290.35335689045936397No Hit
AACGTAGGGTGCAAGCGTTGTCCGGAATTACTGGGTGTAAAGGGAGCGCA1260.3451392883556578No Hit
TACGTAGGGAGCGAGCGTTGTCCGGAATTACTGGGTGTAAAGGGAGCGCA1050.28761607362971486No Hit
TACGTATGGAGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGAGCGCA1000.2739200701235379No Hit
TACGTAGGGAGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGCGCGCA980.2684416687210672No Hit
TACGTATGGTGCAAGCGTTATCCGGCTTTACTGGGTGTAAAGGGAGCGCA900.24652806311118414No Hit
TACGGAGGATCCGAGCGTTATCCGGCTTTATTGGGTTTAAAGGGAGCGTA830.2273536582025365No Hit
TACGTAGGGGGCAAGCGTTATCCGGCTTTACTGGGTGTAAAGGGAGCGTA790.21639685539759498No Hit
TACGGAGGATCCGCGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTA700.19174404908647655No Hit
TACGTATGGAGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGAGCGTA660.18078724628153506No Hit
TACGTAGGTGGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGCGTGTA620.16983044347659354No Hit
TACGTATGGTGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGAGTGTA610.16709124277535817No Hit
AACGTAGGTCACAAGCGTTGTCCGGACTTACTGGGTGTAAAGGGAGCGCA550.15065603856794588No Hit
TACGTAGGGGGCAAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTA540.1479168378667105No Hit
TACGTAGGGGGCAAGCGTTATCCGGATTTACTGGGTGCAAAGGGAGCGTA520.14243843646423973No Hit
TACGTAGGTGGCAAGCGTTATCCGGAATCATTGGGCGTAAAGGGTGCGTA520.14243843646423973No Hit
TACGGAGGATCCGAGCGTTATCCGGATTTACTGGGTGTAAAGGGAGCGTA490.1342208343605336No Hit
AACGTAGGTCACAAGCGTTGTCCGGAATTACTGGGTGCAAAGGGAGCGCA480.1314816336592982No Hit
TACGTAGGTCACAAGCGTTGTCCGGAATTACTGGGTGTAAAGGGAGCGCA460.12600323225682744No Hit
TACGTAGGGGGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGTGCGTA450.12326403155559207No Hit
TACGGAGGATCCGAGCGTTATCCGGATTTCTTGGGTTTAAAGGGAGCGTA450.12326403155559207No Hit
TACGTAGGGGGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGAGTGTA450.12326403155559207No Hit
TACGTATGGTGCAAGCGTTATCCGGATTTACTGGGTGCAAAGGGAGCGCA440.1205248308543567No Hit
TACGTAGGTGGCAAGCGTTGTCCGGATTTACTGGGCGTAAAGGGAGCGTA400.10956802804941519No Hit
TACGTATGGTGCAAGCGTTGTCCGGAATTACTGGGTGTAAAGGGAGCGCA400.10956802804941519No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATGGGGC100.0022047423213.990426
GTATGGA750.0213.990424
TACGGAT152.586415E-5213.990421
TGACAAG100.0022047423213.990429
TGGAGCA800.0213.990427
ATGGAGC800.0213.990426
AGGTGAC100.0022047423213.990426
GAGCAAG1100.0213.990429
CCGTAGG152.586415E-5213.990422
TACGTAT9600.0213.990421
GTGACAA100.0022047423213.990428
TCCGTAG100.0022047423213.990421
GGGCGAG100.0022047423213.990429
TGGTGCG100.0022047423213.990427
GGAGCAA1100.0213.990428
GGTGCGA100.0022047423213.990428
TAGGTGA100.0022047423213.990425
GTAGGGT1800.0213.99044
TACGTAG12050.0213.10251
CGTAGGG11150.0213.030823
Produced by FastQC (version 0.11.5)