FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005561263

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005561263
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26980
Sequences flagged as poor quality0
Sequence length220
%GC52

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TACGGAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTA449716.66790214974055No Hit
AACGTAGGTCACAAGCGTTGTCCGGAATTACTGGGTGTAAAGGGAGCGCA360013.343217197924389No Hit
TACGTAGGGGGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGAGCGTA352813.0763528539659No Hit
TACGTATGGTGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGAGCGCA298211.052631578947368No Hit
TACGTAGGGTGCAAGCGTTATCCGGAATTATTGGGCGTAAAGGGCTCGTA17016.304670126019274No Hit
AACGTAGGGTGCAAGCGTTGTCCGGAATTACTGGGTGTAAAGGGAGCGCA9333.458117123795404No Hit
TACGTAGGGGGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGAGCGCA6932.5685693106004446No Hit
TACGTATGGAGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGAGTGTA6432.383246849518162No Hit
TACGTATGGTGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGAGCGTA5992.2201630837657524No Hit
TACGGAGGATTCAAGCGTTATCCGGATTTATTGGGTTTAAAGGGTGCGTA5381.9940696812453669No Hit
TACGTAGGGGGCAAGCGTTATCCGGAATTACTGGGTGTAAAGGGTGCGTA4991.849518161601186No Hit
TACGTAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGTGCGCA4861.8013343217197924No Hit
TACGTAGGGAGCGAGCGTTGTCCGGATTTACTGGGTGTAAAGGGTGCGTA4071.508524833209785No Hit
TACGTAGGGAGCGAGCGTTGTCCGGAATTACTGGGTGTAAAGGGAGCGTA2921.0822831727205338No Hit
TACGTATGGAGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGTGCGTA2881.0674573758339512No Hit
TACGTAGGTGGCAAGCGTTGTCCGGAATTATTGGGCGTAAAGCGCGCGCA1740.6449221645663454No Hit
TACGGAGGATGCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGTGCGTA1660.6152705707931801No Hit
TACGTAGGGGGCGAGCGTTATCCGGATTCATTGGGCGTAAAGCGCGCGTA1610.5967383246849518No Hit
TACGTAGGTGGCAAGCGTTGTCCGGATTTACTGGGTGTAAAGGGCGTGCA1470.5448480355819125No Hit
TACGGAGGATGCAAGCGTTATCCGGATTTATTGGGTTTAAAGGGTGCGTA1430.5300222386953298No Hit
TACGTATGGAGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGAGCGTA1340.49666419570051895No Hit
TACGGAGGATCCAAGCGTTATCCGGATTTATTGGGTTTAAAGGGTGCGTA1230.4558932542624166No Hit
TACGTAGGGGGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGTGCGTA1050.3891771682727947No Hit
TACGTAGGATGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGAGCGCA1020.37805782060785764No Hit
TACGTAGGGAGCAAGCGTTATCCGGATTTATTGGGTGTAAAGGGTGCGTA1010.374351371386212No Hit
TACGTAGGGGGCAAGCGTTATCCGGAATTACTGGGTGTAAAGGGAGCGTA970.35952557449962935No Hit
TACGGAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGTGCGTA900.3335804299481097No Hit
TACGTATGGAGCAAGCGTTATCCGGATTTATTGGGCGTAAAGCGCGCGTA890.329873980726464No Hit
TACGTAGGTGGCAAGCGTTGTCCGGATTTACTGGGTGTAAAGGGCGTGTA860.31875463306152707No Hit
TACGTAGGGAGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGCGCGCA810.3002223869532987No Hit
TACGTATGGAGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGAGCGCA770.2853965900667161No Hit
TACGTATGGCGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGAGTGTA710.2631578947368421No Hit
TACGTATGGTGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGTGCGTA690.25574499629355074No Hit
TACGTAGGTGGCGAGCGTTATCCGGAATTATTGGGCGTAAAGAGGGAGCA680.2520385470719051No Hit
AACGTAGGTCACAAGCGTTGTCCGGAATTACTGGGTGTAAAGGGAGCGTA610.22609340252038546No Hit
TACGTAGGGGGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGAGTGTA600.2223869532987398No Hit
TACGTAGGTGACAAGCGTTGTCCGGATTTACTGGGTGTAAAGGGCGCGTA590.21868050407709413No Hit
TACGTAGGGCGCAAGCGTTATCCGGATTTATTGGGCGTAAAGGGCTCGTA500.18532246108228317No Hit
TACGTAGGGAGCGAGCGTTGTCCGGAATTACTGGGTGTAAAGGGAGCGCA500.18532246108228317No Hit
TACGTAGGGGGCGAGCGTTGTCCGGAATGATTGGGCGTAAAGGGCGCGTA350.12972572275759822No Hit
TACGTAGGGCGCGAGCGTTGTCCGGAATTACTGGGTGTAAAGGGAGCGTA340.12601927353595255No Hit
TACGTATGGTGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGAGTGTA310.11489992587101557No Hit
TACGTAGGGTGCAAGCGTTGTCCGGAATTACTGGGTGTAAAGGGAGCGCA300.1111934766493699No Hit
TACGTATGGCGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGTGCGTA300.1111934766493699No Hit
TACGTAGGGGGCTAGCGTTATCCGGAATTACTGGGCGTAAAGGGTGCGTA290.10748702742772423No Hit
TACGTAGGGCGCGAGCGTTGTCCGGATTTACTGGGTGTAAAGGGTGCGTA290.10748702742772423No Hit
TACGTAGGGTGCAAGCGTTACTCGGAATTACTGGGCGTAAAGCGTGCGCA290.10748702742772423No Hit
TACGTAGGGAGCGAGCGTTATCCGGATTTACTGGGTGTAAAGGGCGTGTA280.10378057820607858No Hit
TACGGGGGATGCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGCGCGTA280.10378057820607858No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGGGGC4700.0214.000026
GAGGATT450.0214.000025
AGGATTC450.0214.000026
AGGATGC450.0214.000026
GATTCAA450.0214.000028
ATTCAAG450.0214.000029
GTATGGA1350.0214.04
GGATGCA304.1836756E-11214.07
TAGGATG100.0022012526214.05
GAGGATG350.0214.05
ATCCAAG253.5288394E-9214.09
GATGCAA350.0214.08
ATGGTGC4600.0214.06
TGACAAG100.0022012526214.09
GGATCCA253.5288394E-9214.07
ATGCGAG203.0108458E-7214.09
CGTAGGG9500.0214.03
CGTAGGA100.0022012526214.03
ATGGAGC1350.0214.06
GGGAGCA152.5784591E-5214.07
Produced by FastQC (version 0.11.5)