FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005561286

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005561286
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences30352
Sequences flagged as poor quality0
Sequence length219
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTGTTTGATCCCCATGCTTTCGTGCCTCAGCGTCAGTTGACAGCCAGAT634420.901423299947286No Hit
CCTGTTTGATACCCACACTTTCGAGCCTCAATGTCAGTTGCAGCTTAGCA601919.83065366367949No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGTGTCAGTTACAGTCCAGTG25088.263046916183448No Hit
CCTGTTTGATACCCACACTTTCGAGCATCAGTGTCAGTTGCAGTCTAGTG18776.184106483921982No Hit
CCTGTTTGATACCCACACTTTCGAGCATCAGTGTCAGTTGCAGTCCAGTG18416.065498154981549No Hit
CCTGTTTGATACCCACACTTTCGAGCATCAGCGTCAGTTACACTCCAGTG18215.9996046389035325No Hit
CCTGTTTGCTACCCACACTTTCGAGCCTCAGCGTCAGTTGGTGCCCAGTA14114.648787559304164No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTTACAGTCCAGTA14074.63560885608856No Hit
CCTGTTTGATCCCCATGCTTTCGAGCCTCAGCGTCAATATACAGCCAGAT7452.4545334739061673No Hit
CCTGTTTGATACCCACACTTTCGAGCATCAGCGTCAGTTACAGTCCAGCA4751.5649710068529257No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTTACCGTCCAGTA4311.4200052714812863No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTTACTGTCCAGTA3471.143252503953611No Hit
CCTGTTTGATCCCCACGCTTTCGTGCATCAGCGTCAGTCATGGCTTGGCA2950.9719293621507644No Hit
CCTGTTCGATACCCACGCTTTCGTGCCTCAGCGTCTGTCGCACCCTAGCA2910.9587506589351609No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTATCGTCCAGTA2700.8895624670532419No Hit
CCTGTTTGATACCCACACTTTCGAGCATCAGCGTCAGTTACAGTCCAGTA2260.7445967316816026No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGTGTCAGTTACAGTCCAGTA1880.6193990511333685No Hit
CCTGTTTGCTCCCCACACTTTCGCGCCTCACCGTCAGTTATCGTCCAGCA1570.5172641012124407No Hit
CCTGTTCGCTACCCACGCTCTCGTGCATCAGCGTCAGTTACAGTCTGGTA1410.46454928835002635No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAACGTCAGATATAGTTTGGTA1350.44478123352662097No Hit
CCTGTTTGATCCCCACGCTTTCGTGCTTCAGTGTCAGTTATGGTTTAGTA1310.4316025303110174No Hit
CCTGTTCGCTACCCACGCTCTCGTGCTTCAGCGTCAGTTACAGTCTGGTA1240.4085397996837111No Hit
CCTGTTTGATACCCACACTTTCGAGCATCAGTGTCAGTAACAGTCTAGTG1220.40195044807590935No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTGAGCGTCAGTTATGTCCCAGGA1100.36241433842909854No Hit
CCTGTTTGATCCCCATGCTTTCGTGCCTCAGCGTCAGTTGACCGCCAGAT900.2965208223510807No Hit
CCTGTTCGCTACCCACGCTCTCGCGCATCAGCGTCAGATCGAGCCTGGGA870.28663679493937794No Hit
CCTGTTTGATCCCCATGCTTTCGAGCCTCAGCGTCACTATACAGCCAGAT840.27675276752767525No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTAATCGTCCAGTA780.25698471270426987No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAGTGTCAGTTTCAGTCCAGTA700.23062730627306272No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTTCAGTCCAGAA630.20756457564575648No Hit
CCTGTTTGATACCCACACTTTCGAGCCTCAGTGTCAGTTGCAGTCTAGTG540.1779124934106484No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTAAAGCCCAGTA510.1680284659989457No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTTACAGTCCAGTG460.15155508697944123No Hit
CCTGTTTGCTACCCACGCTTTCGCACCTCAGCGTCAGTTACCGTCCAGGT440.14496573537163943No Hit
CTGTTTGATCCCCATGCTTTCGTGCCTCAGCGTCAGTTGACAGCCAGATA420.13837638376383762No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTTGCCGTCCAGTA410.13508170795993674No Hit
CCTGTTTGCTACCCATGCTTTCGAGCCTCAGCGTCAGTTGGTGCCCAGTA400.13178703215603585No Hit
CCTATTTGCTCCCCACGCTTTCGGGACTGAGCGTCAGTTATGCGCCAGAT390.12849235635213493No Hit
CTGTTTGATACCCACACTTTCGAGCCTCAATGTCAGTTGCAGCTTAGCAG330.10872430152872956No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTGGTGCCCAGTA330.10872430152872956No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCTATTT100.0022337155213.01
CGATACC253.6416168E-9213.07
TCGATAC253.6416168E-9213.06
CCTGTTC600.0213.01
TTCGATA253.6416168E-9213.05
TGTTCGA253.6416168E-9213.03
CGCTACC400.0213.07
GCTACCC2100.0213.08
GATCCCT100.0022337155213.08
TATTTGC100.0022337155213.03
ATTTGCT100.0022337155213.04
TGCTACC1700.0213.07
CTGTTCG600.0213.02
TTTGCTA1750.0213.05
CTATTTG100.0022337155213.02
TTGCTAC1750.0213.06
GTTCGCT400.0213.04
ATCCCTA100.0022337155213.09
GTTCGAT253.6416168E-9213.04
TGTTCGC350.0212.999983
Produced by FastQC (version 0.11.5)