FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005561369

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005561369
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences50027
Sequences flagged as poor quality0
Sequence length220
%GC52

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TACGTATGGTGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGAGCGCA1426428.512603194275094No Hit
AACGTAGGTCACAAGCGTTGTCCGGAATTACTGGGTGTAAAGGGAGCGCA790215.79547044595918No Hit
TACGTAGGGGGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGAGCGTA571611.425830051772042No Hit
TACGTATGGAGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGAGTGTA522610.446358966158273No Hit
TACGGAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTA37017.398005077258281No Hit
TACGTATGGTGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGAGCGTA12742.546624822595798No Hit
TACGTAGGGGGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGAGCGCA12292.4566733963659626No Hit
TACGTAGGGTGCAAGCGTTATCCGGAATTATTGGGCGTAAAGGGCTCGTA8581.7150738601155378No Hit
TACGTATGGAGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGAGCGCA6431.2853059347952107No Hit
TACGTAGGGGGCAAGCGTTATCCGGAATTACTGGGTGTAAAGGGTGCGTA6331.265316728966358No Hit
TACGTATGGCGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGAGTGTA6041.2073480320626861No Hit
TACGTATGGTGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGAGTGTA5321.0634257500949489No Hit
TACGTATGGAGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGTGCGTA2940.5876826513682611No Hit
TACGGAGGGTGCGAGCGTTAATCGGAATAACTGGGCGTAAAGGGCACGCA2560.511723669218622No Hit
TACGTAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGTGCGCA2450.48973554280688425No Hit
TACGTAGGGGGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGAGTGTA2380.4757430987266876No Hit
TACGTAGGGAGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGCGCGCA2320.46374957522937615No Hit
TACGTATGGAGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGAGCGTA2180.43576468706898275No Hit
AACGTAGGGTGCAAGCGTTGTCCGGAATTACTGGGTGTAAAGGGAGCGCA1810.3618046255022288No Hit
TACGTAGGTGACAAGCGTTGTCCGGATTTACTGGGTGTAAAGGGCGCGTA1650.32982189617606494No Hit
TACGTAGGGGGCTAGCGTTATCCGGAATTACTGGGCGTAAAGGGTGCGTA1290.2578607551921962No Hit
TACGTAGGGGGCTAGCGTTATCCGGATTTACTGGGCGTAAAGGGTGCGTA1210.24186939052911427No Hit
TACGTAGGTGGCGAGCGTTATCCGGAATTATTGGGCGTAAAGAGGGAGCA1160.23187478761468808No Hit
TACGTAGGGAGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGCGAGTA1000.1998920582885242No Hit
TACGTAGGGAGCGAGCGTTGTCCGGAATTACTGGGTGTAAAGGGAGCGTA800.15991364663081936No Hit
TACGTATGGGGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGAGCGTA790.1579147260479341No Hit
TACGTAGGTCCCGAGCGTTGTCCGGATTTATTGGGCGTAAAGCGAGCGCA730.14592120255062266No Hit
ACGTATGGTGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGAGCGCAG710.14192336138485218No Hit
AACGTAGGTCACAAGCGTTGTCCGGAATTACTGGGTGTAAAGGGAGCGTA700.13992444080196692No Hit
TACGTATGGCGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGAGCGCA670.1339276790533112No Hit
TACGTATGGTGCAAGCGTTGTCCGGAATTACTGGGTGTAAAGGGAGCGCA650.12992983788754073No Hit
TACGGATGGTGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGAGCGCA590.11793631439022928No Hit
TACGTATGGTGCAAGCGTTATCCGGCTTTACTGGGTGTAAAGGGAGCGCA580.11593739380734404No Hit
TACGTAGGTCACAAGCGTTGTCCGGAATTACTGGGTGTAAAGGGAGCGCA580.11593739380734404No Hit
TACGTAGGGGGCGAGCGTTATCCGGATTCATTGGGCGTAAAGCGCGCGTA570.11393847322445878No Hit
TACGTATGGGGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGAGCGCA570.11393847322445878No Hit
TACGTAGGGGGCGAGCGTTATCCGGATTTACTGGGTGTAAAGGGAGCGTA560.11193955264157354No Hit
AACGTAGGTCACAAGCGTTATCCGGATTTACTGGGTGTAAAGGGAGCGCA550.1099406320586883No Hit
TACGTAGGGAGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGAGCGCA530.10594279089291782No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGGTCA152.5916703E-5214.05
AACGTAG8700.0214.01
GGTGACA304.1836756E-11214.07
GTCACAA8550.0214.08
GGTCACA8600.0214.07
AGGTGAC304.1836756E-11214.06
CCGTAGG152.5916703E-5214.02
GGGGGCA8900.0214.07
GTGACAA304.1836756E-11214.08
GGAGGTC152.5916703E-5214.04
GTAGGGA650.0214.04
GTGCGAG304.1836756E-11214.09
AACGGAG152.5916703E-5214.01
GGTGCGA304.1836756E-11214.08
TATGGCG850.0214.05
TAGGTGA304.1836756E-11214.05
GGATGGT100.0022068822213.999984
GGTCCCG100.0022068822213.999987
TACGGAT100.0022068822213.999981
TACGTAG11850.0213.999981
Produced by FastQC (version 0.11.5)