FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005561430

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005561430
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18584
Sequences flagged as poor quality0
Sequence length219
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTGTTTGCTACCCACACTTTCGAGCCTCAGCGTCAGTTGGTGCCCAGTA350718.871071889797676No Hit
CCTGTTCGATCCCCACGCTTTCGTGCCTCAGCGTCAGTCGGGCGCCGGTA226812.204046491605682No Hit
CCTGTTTGCTACCCATGCTTTCGAGCCTCAGCGTCAGTTGGTGCCCAGTA9525.122686181661645No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAACGTCAGATATAGTTTGGTA8884.778303917348257No Hit
CCTGTTTGATACCCACACTTTCGAGCCTCAATGTCAGTTGCAGCTTAGCA6923.7236332328885062No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTTACCGTCCAGTA5532.975678002582867No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCATGAGCGTCAGTCATGTCCCAGAG5482.9487731381833835No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTATCGTCCAGTA5432.9218682737839No Hit
CCTGTTTGCTCCCCACGCTTTCGTGTCTCAGCGTCAGTATTGGCCTAGTG5132.7604390873869997No Hit
CCTGTTTGATACCCACACTTTCGAGCATCAGCGTCAGTTACAGTCCAGCA4212.2653895824365047No Hit
CCTGTTTGATACCCACACTTTCGAGCCTCAACGTCAGTTACAGCTTAGCA3772.02862677572105No Hit
CCTGTTTGATACCCACACTTTCGAGCATCAATGTCAGTTACAGGTTAGTA3631.9532931554024968No Hit
CCTGTTTGCTACCCACGCTTTCGTGCCTCAGCGTCAGTTAAAGCCCAGCA3181.7111493758071459No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCACCGTCAGTTGTCGTCCAGCA3021.6250538097287988No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTTACAGTCCAGTA2811.5120533792509687No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAGTGTCAGTTTCAGTCCAGTA2461.3237193284545847No Hit
CCTGTTCGCTCCCCACGCTTTCGCTCCTCAGCGTCAGTAACGGCCCAGAG2401.2914334911752046No Hit
CCTGTTTGATACCCACACTTTCGAGCATCAGCGTCAGTTACAGTCCAGTA2231.1999569522169609No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTACAGTCCAGTA1590.8555746879035729No Hit
CCTGTTTGATACCCGCACTTTCGAGCCTCAGCGTCAGTTATGCCCCGGCA1540.8286698235040895No Hit
CCTGTTTGATACCCGCACTTTCGAGCCTCAGCGTCAGTGCTGCCCCGGCA1230.6618596642272924No Hit
CCTGTTTGCTCCCCACACTTTCGCGCCTCACCGTCAGTTGCTGTCCAGCA1160.6241928540680155No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTTGTCGTCCAGCA1090.5865260439087386No Hit
CCTGTTTGCTCCCCACACTTTCGCGCCTCACCGTCAGTTATCGTCCAGCA1080.581145071028842No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAACGTCAGCTATAGTTTGGTA990.5327163151097719No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTTACTGTCCAGTA990.5327163151097719No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAGTGTCAGTAGCAGTCCAGTA960.5165733964700818No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGTGTCAGTTACAGTCCAGTG960.5165733964700818No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTCATCGTCCAGCA870.4681446405510116No Hit
CCTGTTTGATACCCACACTTTCGAGCATGAACGTCAGTTACAGTTTAGCA800.4304778303917348No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTGGTGCCCAGTA780.4197158846319414No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTTTTCGTCCAGAA770.41433491175204473No Hit
CCTGTTTGCTCCCCACACTTTCGAGCCTCAGCGTCAGTTAAAGCCCAGCA770.41433491175204473No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTACCGTCCAGTA700.376668101592768No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGTGTCAGTTACAGTCCAGTA680.3659061558329746No Hit
CCTGTTTGATACCCACACTTTCGAGCATCAGCGTCAGTTACACTCCAGTG660.35514421007318125No Hit
CCTCTTCGATCCCCTAGCTTTCGTTCTTGATTAATGAAAACATCCTTGGT600.32285837279380114No Hit
CCTGTTCGCTCCCCACGCTTTCGCTCCTCAGCGTCAGTGACGGCCCAGAG570.30671545415411106No Hit
CCTGTTTGCTCCCCACACTTTCGAGCCTCAGCGTCAGTTAAAGCCCAGTT540.290572535514421No Hit
CCTGTTTGCTCCCCACACTTTCGTGTCTCAGCGTCAGGTATCGTCCAGTA540.290572535514421No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTAAAAGCCCAGCA540.290572535514421No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTTAATGTCCAGCA520.27981058975462764No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAATGTCAGTTACAGTCCAGTA520.27981058975462764No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTTATCGTCCAGTT520.27981058975462764No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTAATCGTCCAGTA510.27442961687473094No Hit
CCTGTTTGATACCCACACTTTCGAGCATCAGTGTCAGTTGCAGTCTAGTG490.26366767111493755No Hit
CCTGTTTGATACCCACACTTTCGAGCATCAGCGTCAGTTACAATCCAGTA490.26366767111493755No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCATTGTCAGTCCAGTA480.2582866982350409No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTTGCCGTCCAGTA480.2582866982350409No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTCATCGTCCAGTA470.2529057253551442No Hit
CCTGTTTGATCCCCACGCTTTCGTGCTTCAGTGTCAGTTATGGTTTAGTA420.2260008609556608No Hit
CCTGTTTGCTCCCCACACTTTCGTGTCTCAGCGTCAGTAATCGTCCAGTA420.2260008609556608No Hit
CCTGTTTGCTCCCCACGCTTTCGTGTCTCAGCGTCAGTTGTGGTCCAGTA400.2152389151958674No Hit
CCTGTTTGCTACCCACGCTTTCGCACCTCAGCGTCAGTTACCGTCCAGGT390.2098579423159707No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCACCGTCAGTTACCGTCCAGTA390.2098579423159707No Hit
CCTGTTTGATCCCCACGCTTTCGTGCATCAGCGTCAGTCATGGCTTGGCA390.2098579423159707No Hit
CCTGTTTGCTCCCCATGCTTTCGTGCCTCAGCGTCAGTTAAAGCCCAGCA370.19909599655617732No Hit
CCTGTTTGCTCCCCACACTTTCGAGCCTCAGCGTCAGTAAAAGCCCAGTT370.19909599655617732No Hit
CTGTTTGCTACCCACACTTTCGAGCCTCAGCGTCAGTTGGTGCCCAGTAG360.19371502367628068No Hit
CCCTTTCGCTCCCCTGGCCTTCGTGCCTCAGCGTCAGTTAATGTCCAGGA350.188334050796384No Hit
CCTGTTTGATACCCACACTTTCGAGCATCAGTGTCAGTTGCAGTCCAGTG350.188334050796384No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCATTTACAGTCCAGTA340.1829530779164873No Hit
CCTGTTTGATACCCACACTTTCGAGCATCAGTGTCAGTAACAGTCTAGTG340.1829530779164873No Hit
CCCTTTCGCTCCCCACGCTTTCGAGCATGAGCGTCAGTTTCTGTCCAGTA330.17757210503659063No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTCATCGTCCAGTA320.17219113215669393No Hit
CCTGTTCGCTCCCCACGCTTTCGCGCCTCACCGTCAGTTACCGTCCAGTA310.16681015927679724No Hit
CCTGTTTGCTCCCCACACTTTCGTGCCTCAACGTCAGTTACTGTCCAGAA300.16142918639690057No Hit
CTGTTCGATCCCCACGCTTTCGTGCCTCAGCGTCAGTCGGGCGCCGGTAC270.1452862677572105No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAGTGTCAGTAACAGTCCAGCA270.1452862677572105No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTTGCTGTCCAGCA270.1452862677572105No Hit
CCTGTTTGATACCCACACTTTCGAGCCTCAGCGTCAGTTGGTGCCCAGTA270.1452862677572105No Hit
CCTGTTTGCTCCCCACACTTTCGAGCCTCAGCGTCAGTTGGTGCCCAGTA260.13990529487731382No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCATGAGCGTCAGTTTTGCCCCAGGG250.13452432199741712No Hit
CCTGTTTGCTCCCCACGCTTTCGTACCTCAGTGTCAGTTACAGTCCAGAA240.12914334911752046No Hit
CCTGTTCGCTACCCACGCTCTCGTGCATCAGCGTCAGTTACAGTCTGGTA240.12914334911752046No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGTGTCAGTTACAGTCCAGCA240.12914334911752046No Hit
CCTGTTCGCTCCCCACGCTTTCGTGCCTCAGCGTCAGTAATAGCTTGGAA240.12914334911752046No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAGCGTCAGTCGGGCGCCGGTA230.12376237623762376No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAGTGTCAGTTACAGTCCAGTA210.1130004304778304No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCATGAGCGTCAGTGTTATCCCAGGG200.1076194575979337No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTGAACGTCAGTTACTGTCCAGTA200.1076194575979337No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTTGAT3350.0213.000024
TGTTTGA3350.0213.000023
CGATACC100.0022267278213.07
TCGATAC100.0022267278213.06
GATACCC3300.0213.08
CTCCCCC100.0022267278213.09
TTCGATA100.0022267278213.05
TGATCCC152.6139049E-5213.07
TGTTCGC600.0213.03
CGCTACC152.6139049E-5213.07
CCTTTCG100.0022267278213.02
TTGATCC152.6139049E-5213.06
TTGATAC3200.0213.06
ATACCCG550.0213.09
ATACCCA2750.0213.09
CCCTTTC100.0022267278213.01
GTTCGCT600.0213.04
TTTGATC152.6139049E-5213.05
TTTGATA3200.0213.05
CCTGTTT15200.0212.999981
Produced by FastQC (version 0.11.5)