FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005561560

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005561560
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences38738
Sequences flagged as poor quality0
Sequence length219
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTGTTTGATACCCACACTTTCGAGCATGAACGTCAGTTACAGTTTAGCA414010.687180546233673No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTTACAGTCCAGTA38279.879188393825185No Hit
CCTGTTTGATACCCACACTTTCGAGCCTCAATGTCAGTTGCAGCTTAGCA27257.034436470648975No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTAAAAGCCCAGCA17144.424596003923796No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTATCGTCCAGTA16434.241313439000464No Hit
CCTGTTTGCTACCCACACTTTCGAGCCTCAGCGTCAGTTGGTGCCCAGTA16254.194847436625536No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTTACCGTCCAGTA13623.5159275130362952No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAACGTCAGATATAGTTTGGTA12963.3455521709948886No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCATGAGCGTCAGTCATGTCCCAGAG10232.6408178016417985No Hit
CCTGTTTGATACCCACACTTTCGAGCATCAGCGTCAGTTACAGTCCAGCA8772.2639268934895966No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTTGTCGTCCAGCA7381.9051060973720895No Hit
CCTGTTTGATACCCACACTTTCGAGCATCAGTGTCAGTTGCAGTCCAGTG7101.832825649233311No Hit
CCTGTTTGCTACCCATGCTTTCGAGCCTCAGCGTCAGTTGGTGCCCAGTA5851.5101450771851928No Hit
CCTGTTTGATCCCCATGCTTTCGTGCCTCAGCGTCAGTATATGGCCAGAT5101.316536733956322No Hit
CCTGTTTGCTACCCACACTTTCGTGCCTCAGCGTCAGTTAAAGCCCAGTA4441.1461613919149154No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCACCGTCAGTTGTCGTCCAGCA4131.0661366100469825No Hit
CCTGTTTGATCCCCACGCTTTCGTGCATCAGCGTCAGTCATGGCTTGGCA4011.035159275130363No Hit
CCTGTTTGATCCCCACGCTTTCGTGCTTCAGTGTCAGTTATAGTTTAGTA3991.029996385977593No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTAATGTCCAGAA3640.9396458258041199No Hit
CCCATTTGCTACCCCGGCTTTCGTTCCTCAGTGTCAATTACGGCCCAGTA3590.9267386029221952No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTCATCGTCCAGCA3450.890598378852806No Hit
CCTGTTTGCTCCCCACACTTTCGTGCCTCAACGTCAGTTACAGTCCAGTA3330.8596210439361867No Hit
CCTGTTTGCTCCCCACACTTTCGTGTCTCAGCGTCAGGTATCGTCCAGTA3300.8518767102070319No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAGCGTCAGTTATCGTCCAGTA3300.8518767102070319No Hit
CCTGTTCGATACCCACGCTTTCGTGCCTCAGCGTCTGTTTCACCCTAGCA3040.7847591512210234No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAGCGTCAGTTACAGTCCAGAG2740.707315813929475No Hit
CCTGTTTGATACCCACACTTTCGAGCCTCAACGTCAGTTGCAGCTTAGCA2470.6376168103670814No Hit
CCTGTTTGCTCCCCACACTTTCGCGCCTCACCGTCAGTTGCTGTCCAGCA2290.5911508079921525No Hit
CCTGTTTGATACCCACACTTTCGAGCATCAGCGTCAGTTACAGTCCAGTA2180.562754917651918No Hit
CCTGTTTGCTCCCCACACTTTCGTGCCTCAACGTCAGTTACAGTCCAGAA2180.562754917651918No Hit
CCCATTTGCTACCCTGGCTTTCGTTCCTCAGTGTCAATTACGGCCCAGTA2080.5369404718880686No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTAATCGTCCAGTA2050.5291961381589138No Hit
CCTATTTGCTCCCCACGCTTTCGTGTCTCAGCGTCAGTAATAGCCCAGCA1900.4904744695131395No Hit
CCTGTTTGATCCCCACGCTTTCGTGCTTCAGTGTCAGTTATGGTTTAGTA1760.4543342454437503No Hit
CCTGTTCGATACCCGCGCTTTCGAGCCTCAGCGTCAGTTGCGCTCCGGTA1740.44917135629098043No Hit
CCTGTTTGCTCCCCACACTTTCGCGCCTCAGCGTCAGTTACAGTCCAGTT1730.4465899117145955No Hit
CCTGTTTGATACCCGCACTTTCGAGCCTCAGCGTCAGTTATGCCCCGGCA1710.44142702256182564No Hit
CCCTTTCGCTCCCCTGGCCTTCGTGCCTCAGCGTCAGTTAATGTCCAGGA1670.4311012442562858No Hit
CCTGTTTGATCCCCATGCTTTCGTGCCTCAGCGTCAATCAATGCCCAGAT1610.41561257679797614No Hit
CCTGTTTGCTCCCCACACTTTCGCGCCTCACCGTCAGTTATCGTCCAGCA1530.3949610201868966No Hit
CCTGTTTGCTCCCCACACTTTCGTGCCTCAACGTCAGTTATTGTCCAGAA1510.3897981310341267No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTTTTCGTCCAGAA1510.3897981310341267No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAGCGTCAGTTACAGTCCAGAA1460.37689090815220194No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCTTCAGTGTCAGTTACGGCCCAGTA1450.374309463575817No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGTGTCAGTTACAGTCCAGTA1390.35882079611750733No Hit
CCTGTTTGCTCCCCACACTTTCGTGCCTCAACGTCAGTTACTGTCCAGAA1310.3381692395064278No Hit
CCTGTTTGCTCCCCACACTTTCGAGCCTCAGCGTCAGTTAAAGCCCAGTT1280.33042490577727296No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAGTGTCAGTTACAGTCCAGTA1270.327843461200888No Hit
CCCGTTTGCTCCCCTAGCTTTCGCACTTTAGCGTCAGTAAAGGCCCAGAA1240.3200991274717332No Hit
CCTGTTCGATACCCACGCTTTCGTGCTTCAGCGTCAGTTATAGTATGGTA1240.3200991274717332No Hit
CCTGTTTGATCCCCACGCTTTCGTGCTTCAGCGTCAGTTGAATGCCGGTA1100.2839589034023439No Hit
CCTGTTTGATACCCACACTTTCGAGCATTAACGTCAGTTACAGTTTAGCA1080.27879601424957406No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTTTCAGTCCAGAA1010.26072590221487946No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTGGTGCCCAGTA980.2529815684857246No Hit
CCTGTTTGCTCCCCACACTTTCGTGCCTCAACGTCAGTTTCTGTCCAGAA980.2529815684857246No Hit
CCTGTTTGCTCCCCACGCTTTCGTGTATCAGCGTCAGTTGTGGTCCAGTA920.23749290102741497No Hit
CCTATTTGCTCCCCACGCTTTCGTGCCTCAGCGTCAGTTATGGCCCAGTA860.22200423356910529No Hit
CCTGTTTGATCCCCATGCTTTCGAGCCTCAGCGTCAATATACAGCCAGAT780.2013526769580257No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTTACTGTCCAGTA770.19877123238164077No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAACGTCAGCTATAGTTTGGTA710.1832825649233311No Hit
CCTGTTTGCTCCCCACGCTTTCGTGTCTCAGCGTCAGTTGTGGTCCAGTA690.1781196757705612No Hit
CCTGTTTGCTCCCCACGCTTTCGCACCTGAGCGTCAGTCTTCGTCCAGGG660.17037534204140636No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTCATCGTCCAGTA660.17037534204140636No Hit
CCTGTTTGCTCCCCATGCTTTCGTGCCTCAGCGTCAGTTAAAGCCCAGCA650.1677938974650214No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAGCGTCAGTTGTGCCCCAGTA630.16263100831225152No Hit
CCTGTTTGCTCCCCACGCTTTCGCACCTGAGCGTCAGTCTTTGTCCAGGG610.15746811915948164No Hit
CCTGTTCGCTCCCCACGCTTTCGCTCCTCAGCGTCAGTGACGGCCCAGAG610.15746811915948164No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTTATCGTCCAGTT600.15488667458309668No Hit
CCTGTTTGATACCCACACTTTCGAGCATCAGCGTCAGTTACAATCCAGTA570.14714234085394184No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTTATCGTCCAGTA570.14714234085394184No Hit
CCTGTTTGCTCCCCACACTTTCGTGCCTCAGTGTCAGTAACAGTCCAGAA560.14456089627755692No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAGTGTCAGTTTCAGTCCAGTA560.14456089627755692No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAACGTCAGTTACAGTCCAGAA560.14456089627755692No Hit
CCTGTTCGCTCCCCCAGCTTTCGCGCCTCAGCGTCGGTCTCGGCCCAGAG550.14197945170117196No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTGTCAGTCCAGAA540.13939800712478703No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCATTGTCAGTCCAGTA540.13939800712478703No Hit
CCTGTTTGATCCCCACGCTTTCGTGCCTCAGCGTCAGTTGGGCGCCGGTG540.13939800712478703No Hit
CCTATTTGCTCCCCACGCTTTCGTCCCTGACCGTCAGTGGTGGCCCAGCA520.13423511797201715No Hit
CCTGTTTGCTCCCCATGCTTTCGTCCCTGAGGGTCAGTCATTGTCCAGTG500.12907222881924724No Hit
CCTGTTCGATACCCACGCTTTCGTGCTTCAGCGTCAGTTATAGCCCAGTA500.12907222881924724No Hit
CCTGTTTGCTACCCACGCTTTCGTGCTTCAGTGTCAGTTACGGCCCAGTA490.1264907842428623No Hit
CCTGTTTGCTCCCCACGCTTTCGTCCCTGAGCGTCAGAATAGTCTTCGTC480.12390933966647737No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAGCGTCAGTGTCAGTCCAGAA450.11616500593732253No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAACGTCAGATATAGTTTTGTA430.11100211678455264No Hit
CCTGTTCGCTACCCACGCTCTCGCGCATCAGCGTCAGAACGAGCCTGGGA410.10583922763178273No Hit
CCTGTTTGCTACCCACGCTTTCGTGCCTCAGCGTCAGTTAAAGCCCAGCA400.1032577830553978No Hit
CCTGTTTGATCCCCACGCTTTCGTGCATCAGCGTCAGTCATGGCTTTGCA400.1032577830553978No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)