FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005561640

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005561640
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences38411
Sequences flagged as poor quality0
Sequence length219
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTGTTTGATACCCGCACTTTCGAGCATCAACGTCAGTTACGGTCCAGCA480412.506833979849521No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTAAAAGCCCAGCA464312.08768321574549No Hit
CCTGTTTGCTCCCCACGCTTTCGCACCTCAGCGTCAATACCGGTCCAGGT26056.78191143162115No Hit
CCTGTTTGATACCCACACTTTCGAGCCTCAACGTCAGTCGCGGCTTAGCA18794.891827861810419No Hit
CCTGTTTGATACCCACACTTTCGAGCCTCAATGTCAGTTGCAGCTTAGCA15333.991044232121007No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAACGTCAGATATAGTTTGGTA10332.689333784592955No Hit
CCTGTTTGCTACCCACACTTTCGAGCCTCAGCGTCAGTTGGTGCCCAGTA10152.642472208481945No Hit
CCTGTTTGATACCCACACTTTCGAGCATCAGCGTCAGTTACAGTCCAGCA10062.61904142042644No Hit
CCTGTTTGATACCCACACTTTCGAGCATCAGTGTCAGTTGCAGTCCAGTG8402.186873551847127No Hit
CCTATTTGCTCCCTGTGCTTTCGTGACTGAGCGTCAGTATTGTGCTGGTA8392.184270130952071No Hit
CCTGTTTGATCCCCACGCTTTCGTGCATCAGCGTCAGTAATGGCTTGGCA7681.9994272474030876No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTTACCGTCCAGTA7041.8328083101194972No Hit
CCTGTTTGATACCCACACTTTCGAGCATCAACGTCAGTTACGCTCCAGCA6051.5750696415089427No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTCACTGTCCAGTA5511.4344849131759132No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCATGAGCGTCAGTCTTGGCCCAGGA5351.3928301788550155No Hit
CCTGTTTGCTCCCCACGCTTTCGCACCTCAGCGTCACTACCGGTCCAGGT5021.306917289318164No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTTACTGTCCAGTA4411.1481086147197417No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTTACAGTCCAGTA4111.0700059878680586No Hit
CCTGTTTGCTACCCATGCTTTCGAGCCTCAGCGTCAGTTGGTGCCCAGCA3891.0127307281768243No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCATGAGCGTCAGTCGCATCCCAGGG3871.0075238863867122No Hit
CCTGTTTGCTACCCACGCTTTCGTGCCTCAGCGTCAGTTAAAGCCCAGCA3720.9684725729608705No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTTAATGTCCAGCA3450.8981802087943558No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAGTGTCAGTTATGGGCCAAAT3230.8409049491031215No Hit
CCTGTTTGATCCCCACGCTTTCGTGCTTCAGTGTCAGTTATGGTTTAGTA3180.827887844627841No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGTGTCAGTTACAGTCCAGTG3100.8070604774673921No Hit
CCTGTTTGCTCCCCACACTTTCGCGCCTCAGCGTCAGTAACAGTCCAGTA3010.7836296894118873No Hit
CCTATTTGCTCCCCACGCTTTCGGGACTGAGCGTCAGCTGCACCCCAGAC2730.7107339043503164No Hit
CCTGTTTGATACCCACACTTTCGAGCATGAACGTCAGTTACGGCTTAGTG2720.7081304834552602No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGTGTCAGTTACAGTCCAGTA2420.6300278566035771No Hit
CCTGTTTGATACCCACACTTTCGAGCATCAGCGTCAGTTACACTCCAGTG2420.6300278566035771No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTATCGTCCAGTA2290.5961833849678477No Hit
CCTGTTTGCTACCCACGCTTTCGCACCTCAGCGTCAGTTTTGTGCCAGGC2150.5597354924370623No Hit
CCTGTTTGATCCCCACGCTTTCGTGCTTCAGTGTCAGTTATAGTTTAGTA1990.5180807581161646No Hit
CCTGTTTGCTACCCATGCTTTCGAGCCTCAGCGTCAGTTGGTGCCCAGTA1970.5128739163260524No Hit
CCTGTTTGCTCCCCACGCTTTCGCACCTGAGCGTCAGTATTTGCCCAGAA1890.4920465491656036No Hit
CCCGTTTGCTACCCTAGCTTTCGTTCCTCAGCGTCAATAACGGCCCAGTG1820.4738226029002109No Hit
CCTGTTTGATACCCACACTTTCGAGCATCAGTGTCAGTTGCAGTCTAGTG1780.4634089193199865No Hit
CCTGTTTGATCCCCACGCTTTCGTGCATCAGCGTCAGTCATGGCTTGGCA1760.4582020775298743No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTTGCTGTCCAGCA1760.4582020775298743No Hit
CCTATTTGCTCCCCACGCTTTCGTGCCTGAGCGTCAGCAACAGTCCAGGC1720.4477883939496498No Hit
CCTGTTCGATACCCACGCTTTCGTGCCTCAGCGTCTGTCGCACCCTAGCA1620.42175418499908884No Hit
CCTGTTTGATACCCACACTTTCGAGCATCAACGTCAGTTACGGTCCAGCA1520.39571997604852777No Hit
CCTGTTCGCTACCCACGCTCTCGCGCATCAGCGTCAGATCGAGCCTGGGA1440.37489260888807896No Hit
CCTGTTCGCTACCCACGCTTTCGTGCATCAGCGTCAGATATAGATTAGCA1420.36968576709796674No Hit
CCTGTTTGCTACCCACACTTTCGTGCCTCAGCGTCAGTTAAAGCCCAGTA1410.3670823462029106No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAACGTCAGCTATAGTTTGGTA1380.3592720835177423No Hit
CCTGTTTGCTACCCATGCTTTCGAGCCTCAGCGTCAGTTAGTGCCCAGTA1320.3436515581474057No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTTACTGTCCAGCA1240.32282419098695686No Hit
CCTGTTTGATACCCACACTTTCGAGCATCAGCGTCAGTTACAGTCCAGTA1140.29678998203639584No Hit
CCTGTTTGATACCCGCACTTTCGAGCCTCAGCGTCAGTTATGCCCCGGCA1120.2915831402462836No Hit
CCTCTTTGCTCCCTGTGCTTTCGTGACTGAGCGTCAGTATTGTGCTGGTA1110.2889797193512275No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTAAAAGCCCAGTA1100.2863762984561714No Hit
CCTGTTTGATCCCCATGCTTTCGTGCCTCAGCGTCAGTATATGGCCAGAT1100.2863762984561714No Hit
CCTGTTTGCTACCCACGCTTTCGTGCATCAGCGTCAATTCTAGACCAGTA1090.2837728775611153No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAGTGTCAGTTACAGTCCAGCA1060.275962614875947No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAGTGTCAGTAACAGTCCAGCA1020.26554893129572255No Hit
CCTGTTCGATACCCACGCTTTCGTGCTTCAGCGTCAGTTATAGTATGGTA1000.2603420895056104No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTTACTGTCCAGCA980.25513524771549817No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTAATCGTCCAGTA920.23951472234516155No Hit
CCTGTTCGCTCCCCTAGCTTTCGCGCCTCAGCGTCAGTGGCGGCCCAGAA890.23170445965999326No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAGCGTCCGATATAGATTTGTA790.20567025070943218No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAACGTCAGTTACAGTCCAGAA790.20567025070943218No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAGCGTCAGTACCAGTCCAGCA780.20306682981437607No Hit
CCCATTTGCTACCCCGGCTTTCGTTCCTCAGTGTCAATTACGGCCCAGTA680.17703262086381505No Hit
CCTGTTTGCTCCCCACGCTTTCGCCCCTCAGCGTCACTACCGGTCCAGGT610.15880867459842232No Hit
CCTGTTTGATACCCTCACTTTCGAGCATCAACGTCAGTTACGGTCCAGCA610.15880867459842232No Hit
CCTGTTTGCTCCCCACACTTTCGCGCCTCAGCGTCAGTTACAGTCCAGTT600.15620525370336624No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCATTTACAGTCCAGTA590.1536018328083101No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAGTGTCAGTTACAGTCCAGTG570.14839499101819792No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTCATCGTCCAGTA570.14839499101819792No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGTGTCAGTTACTGTCCAGTA560.1457915701231418No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCATGAGCGTCAGTTTTGCCCCAGGG550.1431881492280857No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAGTGTCAGTTTCAGTCCAGTA550.1431881492280857No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCACCGTCAGTTGTCGTCCAGTA550.1431881492280857No Hit
CCTGTTTGCTCCCCACACTTTCGTGCCTCAGTGTCAGTTTCAGTCCAGAA530.1379813074379735No Hit
CCTGTTTGATCCCCACGCTTTCGTGCCTCAGCGTCAGTTGGGCGCCGGTG530.1379813074379735No Hit
CCTGTTTGCTACCCACGCTTTCGAGCCTCAGCGTCAGTTACAGCCCAGTA520.1353778865429174No Hit
CCTGTTCGCTACCCACGCTCTCGCGCATCAGCGTCAGAACGAGCCTGGGA500.1301710447528052No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTTATCGTCCAGTA470.12236078206763687No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTGGTGCCCAGTA470.12236078206763687No Hit
CCTGTTTGATCCCCATGCTTTCGTGCCTCAGCGTCAGTTGACAGCCAGAT450.11715394027752467No Hit
CCCATTTGCTCCCCCGGCTTTCGTTCCTCAGCGTCAATAACGGCCCAGTA430.11194709848741245No Hit
CCTGTTTGATACCCGCACTTTCGAGCATCAACGTCAGTTACGCTCCAGCA430.11194709848741245No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTTGTCGTCCAGCA400.10413683580224416No Hit
CCTGTTCGCTCCCCCAGCTTTCGCGCCTCAGCGTCGGTCTCGGCCCAGAG400.10413683580224416No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)