FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005561680

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005561680
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28708
Sequences flagged as poor quality0
Sequence length219
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTGTTTGATACCCACACTTTCGAGCCTCAATGTCAGTTGCAGCTTAGCA672123.411592587432075No Hit
CCTGTTTGATCCCCATGCTTTCGTGCCTCAGCGTCAGTATATGGCCAGAT398313.874181412846593No Hit
CCTGTTTGCTACCCACACTTTCGAGCCTCAGCGTCAGTTGGTGCCCAGTA12154.232269750592169No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAACGTCAGATATAGTTTGGTA11744.0894524174446145No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGTGTCAGTTACAGTCCAGCA11554.023268775254285No Hit
CCTGTTTGATACCCACACTTTCGAGCATCAGCGTCAGTTACAGTCCAGCA9403.274348613626864No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTTACCGTCCAGTA9353.2569318656820396No Hit
CCTGTTTGATACCCACACTTTCGAGCATCAGCGTCAGTTACAGTCCAGTA8352.908596906785565No Hit
CCTGTTTGATACCCACACTTTCGAGCATCAGTGTCAGTTGCAGTCTAGTG7842.7309460777483627No Hit
CCTGTTTGATACCCACACTTTCGAGCATCAGTGTCAGTTGCAGTCCAGTG7212.511495053643584No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTATCGTCCAGTA6692.3303608750174165No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTTACAGTCCAGTA5701.9855092657099067No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGTGTCAGTTACAGTCCAGTG5591.9471924202312945No Hit
CCTGTTTGATCCCCACGCTTTCGTGCTTCAGTGTCAGTTATGGTTTAGTA4721.6441410059913613No Hit
CCTGTTTGCTACCCATGCTTTCGAGCCTCAGCGTCAGTTGGTGCCCAGTA4021.4003065347638288No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGTGTCAGTTACAGTCCAGTA3961.3794064372300403No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTAAAAGCCCAGCA3891.3550229901072872No Hit
CCTGTTTGCTACCCACGCTTTCGTGCATCAGCGTCAATTCTAGACCAGTA3361.1704054618921556No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAGTGTCAGTAGCAGTCCAGTA3221.121638567646649No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCATGAGCGTCAGTCATGTCCCAGAG2360.8220705029956806No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTTTTCGTCCAGAA1920.6688031210812317No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTAATCGTCCAGTA1910.665319771492267No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTACCGTCCAGTA1880.6548697227253727No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTTACTGTCCAGTA1690.5886860805350425No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAGTGTCAGTAACAGTCCAGCA1660.5782360317681482No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAACGTCAGCTATAGTTTGGTA1400.4876689424550648No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAGTGTCAGTTACAGTCCAGTA1310.4563187961543821No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAGTGTCAGTTTCAGTCCAGTA1260.43890204820955836No Hit
CCTGTTTGATACCCACACTTTCGAGCATCAGCGTCAGTTACACTCCAGTG1250.43541869862059357No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGTGTCAGTCACAGTCCAGTG1220.4249686498536993No Hit
CCTGTTTGCTCCCCACACTTTCGCGCCTCACCGTCAGTTATCGTCCAGCA1110.38665180437508706No Hit
CCTGTTTGCTCCCCACACTTTCGTGCCTCAACGTCAGTTATTGTCCAGAA1090.3796851051971576No Hit
CCTGTTTGCTCCCCACGCTTTCGCACATGAGCGTCAGTACATTCCCAAGG930.3239515117737216No Hit
CCTGTTTGCTCCCCACACTTTCGAGCCTCAGCGTCAGTTAAAGCCCAGTT850.2960847150620036No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCACCGTCAGTTGTCGTCCAGCA820.28563466629510936No Hit
CCTGTTTGCTACCCACGCTTTCGTGCCTCAGCGTCAGTTAAAGCCCAGCA820.28563466629510936No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAGCGTCAGTTATGCCCCAGTA770.26821791835028563No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTTGTCGTCCAGCA770.26821791835028563No Hit
CCTGTTCGCTACCCACGCTCTCGCGCATCAGCGTCAGAACGAGCCTGGGA770.26821791835028563No Hit
CCTGTTCGATACCCACGCTTTCGTGCTTCAGCGTCAGTTATAGCCCAGTA750.26125121917235616No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTAAAAGCCCAGTA740.2577678695833914No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTAAAGGCCCAGCA710.24731782081649714No Hit
CTGTTTGATACCCACACTTTCGAGCCTCAATGTCAGTTGCAGCTTAGCAG680.2368677720496029No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTGGTGCCCAGTA610.21248432492684965No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTTATCGTCCAGTT500.17416747944823743No Hit
CCCATTTGCTACCCCGGCTTTCGTTCCTCAGTGTCAATTACGGCCCAGTA490.17068412985927267No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTGAACGTCAGTTACTGTCCAGTA480.16720078027030794No Hit
CCTGTTTGATCCCCATGCTTTCGTGCCTCAGCGTCAGTATATGTCCAGAT470.16371743068134317No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTACAGTCCAGAA440.1532673819144489No Hit
CCTGTTTGCTCCCCACACTTTCGCGCCTCAGCGTCAGTTGTCGTCCAGAA400.13933398355858995No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAATGTCAGTTGCAGCTTAGCA390.1358506339696252No Hit
CCCATTCGCTCCCCCGGCTTTCGTTCCTCAGCGTCAATAACGGCCCAGTA380.13236728438066045No Hit
CTGTTTGATCCCCATGCTTTCGTGCCTCAGCGTCAGTATATGGCCAGATA380.13236728438066045No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCACCGTCAGTTACCGTCCAGTA370.1288839347916957No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTCATCGTCCAGTA360.12540058520273095No Hit
CCTGTTTGCTCCCCACACTTTCGTGCCTCAACGTCAGTTACTGTCCAGAA350.1219172356137662No Hit
CCTGTTCGATACCCACGCTTTCGTGCTTCAGCGTCTGTTTCACCCCAGTA340.11843388602480145No Hit
CCTGTTTGCTACCCACACTTTCGAGCCTCAATGTCAGTTGCAGCTTAGCA330.11495053643583669No Hit
CCCTTTCGCTCCCCACGCTTTCGTGCCTGAGCGTCAGTCCCCTTCCAGTA290.10101713807997771No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTGA100.0022330834213.000023
CCTGTTC100.0022330834213.000021
TGTTCGC100.0022330834213.000023
CCGTTTG100.0022330834213.000022
CCCGTTT100.0022330834213.000021
TCGCTAC100.0022330834213.000026
ATTTGCT100.0022330834213.000024
TCCTGTT100.0022330834213.000021
CTGTTCG100.0022330834213.000022
GTTCGCT100.0022330834213.000024
ATCCCCA5050.0213.09
TGATCCC5050.0213.07
GATCCCC5050.0213.08
TTGATCC5050.0213.06
TTTGATC5050.0213.05
GTTTGAT15100.0212.294714
TGTTTGA15050.0212.292363
CCTGTTT28250.0212.246031
GATACCC10000.0211.935018
ATACCCA10000.0211.935019
Produced by FastQC (version 0.11.5)