FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005561762

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005561762
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences32737
Sequences flagged as poor quality0
Sequence length219
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTGTTTGCTCCCCACGCTTTCGCACCTGAGCGTCAGTCTTCGTCCAGGG2190866.9212206371995No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCTTCAGTGTCAGTTACAGTCCAGTA407512.447689159055503No Hit
CCTGTTTGCTCCCCACGCTTTCGTACCTCAGTGTCAGTTACAGTCCAGCA7172.1901823624644896No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAGTGTCAGTTACAGTCCAGTA6992.135198704829398No Hit
CCTGTTTGCTCCCCACGCTTTCGCACCTTAGCGTCAGTCTTCGTCCAGGG6602.0160674466200326No Hit
CCGGTTCGCGCCCCTAGCTTTCGTCCCTCACCGTCAGAATCGTTCCAGTC4571.3959739744020527No Hit
CCTGTTTGCTCCCCACGCTTTCGCCCCTGAGCGTCAGTCTTCGTCCAGGG4411.347099612059749No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAGTGTCAGTTGCAGGCCAGTA3281.0019244280172281No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCTTCAGTGTCAGTTACGGCCCAGTA2310.7056236063170114No Hit
CCTGTTTGCTACCCACGCTTTCGAGCCTCAGCGTCAGTTATAATCCAGTA2060.6292574151571616No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAGTGTCAGTTACAGTCCAGTG2020.6170388245715857No Hit
CCTGTTCGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTACAGACCAGAG1290.3940495463848245No Hit
CCTGTTTGCTCCCCACGCTTTCGCACCTGAGCGTCAGTCTTCGTCCAGTG1200.3665577175672786No Hit
CTGTTTGCTCCCCACGCTTTCGCACCTGAGCGTCAGTCTTCGTCCAGGGG1180.36044842227449064No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAGTGTCAGTTACGGCCCAGTA730.22298927818676118No Hit
CCTATTTGCTCCCCACGCTTTCGTGACTGAGCGTCAGTTACGGACTGGTA730.22298927818676118No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTACAGTCCAGTA710.2168799828939732No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAGTGTCAGTTACAGTCCAGCA570.17411491584445735No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAACGTCAGATATAGTTTGGTA490.14967773467330542No Hit
CCGGTTCGCGCCCCTAGCTTTCGTCCCTCACCGTCAGCATCGTTCCAGTC480.14662308702691143No Hit
TCCTGTTTGCTCCCCACGCTTTCGCACCTGAGCGTCAGTCTTCGTCCAGG460.14051379173412348No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTTACCGTCCAGTA410.12524055350215352No Hit
CCTGTTTGCTCCCCACGCTTTCGCCCCTTAGCGTCAGTCTTCGTCCAGGG370.11302196291657757No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCGCGC500.0213.000025
CCTGTTT31850.0213.000021
TCGCGCC500.0213.000026
GGTTCGC500.0213.000023
GCGCCCC500.0213.000028
CGGTTCG500.0213.000022
CCGGTTC500.0213.000021
GTTCGCG500.0213.000024
ATCCCCA100.002234506213.09
CCTATTT100.002234506213.01
GTTTGAT152.632215E-5213.04
TGATCCC100.002234506213.07
TGTTTGA152.632215E-5213.03
GCTACCC304.3655746E-11213.08
GATCCCC100.002234506213.08
TTGATCC100.002234506213.06
TTTGCTA304.3655746E-11213.05
TTGCTAC304.3655746E-11213.06
TTTGATC100.002234506213.05
CTGTTTG32000.0212.001572
Produced by FastQC (version 0.11.5)