FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005561764

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005561764
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences37386
Sequences flagged as poor quality0
Sequence length219
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTGTTCGATACCCGCACTTTCGAGCTTCAGCGTCAGTTGCGCTCCAGTG626916.768308992671052No Hit
CCTGTTTGCTACCCACACTTTCGAGCCTCAGCGTCAGTTGGTGCCCAGTA619416.567699138715028No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTTACAGTCCAGTA24926.6655967474455675No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTATCGTCCAGTA14873.977424704434815No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTAAAAGCCCAGCA14673.923928743379875No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAACGTCAGATATAGTTTGGTA14553.8918311667469103No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTTACCGTCCAGTA12493.340822767881025No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAGTGTCAGTAGCAGTCCAGTA12153.249879634087626No Hit
CCTGTTTGATACCCACACTTTCGAGCCTCAATGTCAGTTGCAGCTTAGCA6461.7279195420745734No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTAATCGTCCAGTA6311.6877975712833682No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGTGTCAGTTACAGTCCAGTG6301.685122773230621No Hit
CCTGTTTGATACCCGCACTTTCGAGCCTCAGCGTCAGTCGCACTCCGGGC5751.538008880329535No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCATGAGCGTCAGTCATGTCCCAGAG5251.4042689776921842No Hit
CCTGTTTGCTCCCCACACTTTCGAGCCTCAGCGTCAGTTAGAGCCCAGCA3931.0511956347295779No Hit
CCTGTTTGATACCCACACTTTCGAGCATCAGCGTCAGTTACAGTCCAGCA3871.0351468464130957No Hit
CCTGTTTGCTACCCACGCTTTCGTGCCTCAGCGTCAGTTAAAGCCCAGCA3500.9361793184614562No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCACCGTCAGTTGTCGTCCAGCA3290.8800085593537689No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTTACTGTCCAGTA3010.8051142138768523No Hit
CCTGTTTGCTACCCATGCTTTCGAGCCTCAGCGTCAGTTGGTGCCCAGTA2990.7997646177713582No Hit
CCTGTTTGATACCCACACTTTCGAGCATCAGTGTCAGTTGCAGTCCAGTG2880.7703418391911411No Hit
CCTGTTTGCTCCCCACACTTTCGCGCCTCACCGTCAGTTATCGTCCAGCA2660.7114962820307067No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTACCGTCCAGTA2460.6580003209757663No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAGTGTCAGTTTCAGTCCAGTA2340.6259027443428021No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTTGTCGTCCAGCA2270.607179157973573No Hit
CCTGTTTGATACCCACACTTTCGAGCATGAACGTCAGTTACGGATTAGTG2200.5884555716043438No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGTGTCAGTTACAGTCCAGCA2170.5804311774461027No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGTGTCAGTTACAGTCCAGTA2170.5804311774461027No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTCATCGTCCAGTA2120.5670571871823678No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTAAAGCCCAGTA2030.5429840047076446No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTGGTGCCCAGTA1850.4948376397581983No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAACGTCAGCTATAGTTTGGTA1810.48413844754721014No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAGTGTCAGTTACAGTCCAGTA1790.4787888514417161No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTAAAAGCCCAGTA1780.47611405338896917No Hit
CCTGTTTGCTCCCCACACTTTCGCGCCTCAGCGTCAGTTACAGTCCAGTT1730.4627400631252341No Hit
CCTGTTTGCTCCCCACACTTTCGTGCCTCAACGTCAGTTACTGTCCAGAA1710.45739046701974No Hit
CCTGTTTGATACCCACACTTTCGAGCATCAATGTCAGTTACAGGTTAGTA1680.44936607286149893No Hit
CCTGTTCGATACCCACGCTTTCGAGCCTCAGCGTCAGTCGCGAGTTGGTA1630.43599208259776384No Hit
CCTGTTTGCTCCCCACGCTTTCGCACCTCAGCGTCAATACCGGTCCAGGT1620.4333172845450169No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTCATCGTCCAGCA1540.41191890012304067No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAATGTCAGTTACAGTCCAGTA1370.3664473332263414No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAGTGTCAGTAACAGTCCAGCA1100.2942277858021719No Hit
CCTGTTTGATACCCACACTTTCGAGCATCAGCGTCAGTTACGCTCCAGCA1060.2835285935911839No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTAAAGGCCCAGCA990.26480500722195477No Hit
CCTGTTTGATCCCCACGCTTTCGTGCATCAGCGTCAGTAATGGCTTGGCA980.26213020916920776No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTTAATGTCCAGCA910.2434066227999786No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCATTTACAGTCCAGTA860.2300326325362435No Hit
CCTGTTTGATACCCACACTTTCGAGCATCAGCGTCAGTTACAATCCAGTA850.2273578344834965No Hit
CCTGTTCGATACCCTCACTTTCGAGCTTCAGCGTCAGTTGCGCTCCAGTG850.2273578344834965No Hit
CTGTTCGATACCCGCACTTTCGAGCTTCAGCGTCAGTTGCGCTCCAGTGA830.22200823837800246No Hit
CCTATTTGCTCCCCACGCTTTCGTGCTTCAGTGTCAGAATCCAGACCAGA820.21933344032525545No Hit
CCTGTTTGATACCCACACTTTCGAGCATCAGCGTCAGTTACAGTCCAGTA810.21665864227250844No Hit
CCTATTTGCTCCCCACGCTTTCGGGACTGAGCGTCAGTTATGCGCCAGAT810.21665864227250844No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTGAACGTCAGTTACTGTCCAGTA780.20863424811426737No Hit
CTGTTTGCTACCCACACTTTCGAGCCTCAGCGTCAGTTGGTGCCCAGTAG770.20595945006152033No Hit
CCCGTTTGCTCCCCTAGCTTTCGCACTTTAGCGTCAGTAAAGGCCCAGAA770.20595945006152033No Hit
CCTGTTTGATACCCGCACTTTCGAGCCTCAGCGTCAGTTATGCCCCGGCA730.19526025785053228No Hit
CCTGTTCGCTACCCACGCTCTCGCGCATCAGCGTCAGATCGAGCCTGGGA690.1845610656395442No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTTTCAGTCCAGAA690.1845610656395442No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTCATCGTCCAGTA670.17921146953405018No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTTGCCGTCCAGTA670.17921146953405018No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCATTGTCAGTCCAGTA660.17653667148130317No Hit
CCTGTTTGCTCCCCACACTTTCGAGCCTCAGCGTCAGTTGGTGCCCAGTA610.16316268121756808No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTGAGCGTCAGTTATGTCCCAGGA610.16316268121756808No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAGTGTCAGTCACAGTCCAGTA600.16048788316482104No Hit
CCTGTTTGCTACCCACACTTTCGAGCCTCAGCGTCAGTTGGTGCCCAGCA580.15513828705932703No Hit
CCTGTTTGCTCCCCACACTTTCGAGCCTCAGCGTCAGTAAAAGCCCAGTT550.14711389290108595No Hit
CCTGTTTGATACCCACACTTTCGAGCATGAACGTCAGTTACGGCTTAGTG520.1390894987428449No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTGAACGTCAGTTACTGTCCAGCA510.1364147006900979No Hit
CCTGTTTGCTCCCCACACTTTCGTGTCTCAGCGTCAGGTATCGTCCAGTA470.1257155084791098No Hit
CCTGTTCGATACCCACGCTTTCGTGCCTCAGCGTCTGTCGCACCCTAGCA470.1257155084791098No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTGTCGTCCAGTA460.1230407104263628No Hit
CCTGTTTGATACCCGCACTTTCGAGCTTCAGCGTCAGTTGCGCTCCAGTG410.10966672016262773No Hit
CCTGTTCGATACCCGCACTTTCGAGCTTCAGCGTCAGTTACGCTACTGCA410.10966672016262773No Hit
CCTGTTCGCTACCCACGCTCTCGTGCATCAGCGTCAGTTACAGTCTGGTA380.10164232600438666No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGTTCGC500.0213.000023
CGCTACC253.6579877E-9213.000027
GTTCGCT500.0213.000024
CGTTTGC100.0022357765213.03
ATCCCCA152.635211E-5213.09
CCTATTT203.0978845E-7213.01
CTCCCCC100.0022357765213.09
TGATCCC152.635211E-5213.07
CCGTTTG100.0022357765213.02
CCCGTTT100.0022357765213.01
GATCCCC152.635211E-5213.08
TATTTGC203.0978845E-7213.03
TTGATCC152.635211E-5213.06
ATTTGCT203.0978845E-7213.04
ATACCCA2550.0213.09
TCCTGTT304.3655746E-11213.01
CTATTTG203.0978845E-7213.02
TTTGATC152.635211E-5213.05
GCTACCC7550.0211.58948
TGCTACC7300.0211.541097
Produced by FastQC (version 0.11.5)