FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005561771

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005561771
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17582
Sequences flagged as poor quality0
Sequence length220
%GC51

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AACGTAGGTCACAAGCGTTGTCCGGAATTACTGGGTGTAAAGGGAGCGCA351820.009100216130133No Hit
TACGGAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTA261414.867478102604936No Hit
TACGTAGGGGGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGAGCGTA182310.36855875327039No Hit
TACGTAGGTGGCAAGCGTTGTCCGGATTTACTGGGTGTAAAGGGCGTGTA8064.584233875554544No Hit
TACGTAGGGGGCAAGCGTTATCCGGAATTACTGGGTGTAAAGGGTGCGTA5192.951882607211921No Hit
TACGTAGGTGGCAAGCGTTGTCCGGATTTACTGGGTGTAAAGGGCGTGCA4822.7414401092025935No Hit
TACGTATGGTGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGAGCGCA4432.5196223410305993No Hit
TACGTAGGGAGCAAGCGTTGTCCGGATTTACTGGGTGTAAAGGGTGCGTA4422.5139347059492665No Hit
TACGTATGGAGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGAGTGTA3481.9792970083039472No Hit
TACGTAGGTGGCAAGCGTTGTCCGGAATTACTGGGTGTAAAGGGAGTGTA3111.7688545102946196No Hit
TACGTAGGTGGCAAGCGTTGTCCGGAATTACTGGGTGTAAAGGGAGCGCA2401.3650324195199637No Hit
TACGGAGGATTCAAGCGTTATCCGGATTTATTGGGTTTAAAGGGTGCGTA2111.2000910021613014No Hit
AACGTAGGGTGCAAGCGTTGTCCGGAATTACTGGGTGTAAAGGGAGCGCA2061.1716528267546353No Hit
TACAGAGGTCTCAAGCGTTGTTCGGAATCACTGGGCGTAAAGGGTACGTA1500.8531452621999773No Hit
TACGTAGGTGGCAAGCGTTGTCCGGAATTATTGGGCGTAAAGCGCGCGCA1330.7564554658173132No Hit
TACGTAGGGTGCGAGCGTTAATCGGAATTACTGGGCGTAAAGGGTGCGCA1290.7337049254919804No Hit
TACGTAGGGGGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGAGCGCA1250.7109543851666478No Hit
TACGGAGGATGCAAGCGTTATCCGGATTTATTGGGTTTAAAGGGTGCGTA1180.6711409395973155No Hit
TACGTATGGTGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGAGCGTA1160.659765669434649No Hit
TACGTAGGGGGCGAGCGTTGTCCGGAATTACTGGGCGTAAAGGGAGCGTA1070.6085769537026504No Hit
TACGTAGGTGGCAAGCGTTGTCCGGATTTACTGGGTGTAAAGGGCGCGTA1040.591514048458651No Hit
TACGGAGGATGCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGTGCGTA770.4379479012626549No Hit
TACGTATGGAGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGTGCGTA760.4322602661813218No Hit
TACGTAGGGGGCGAGCGTTGTCCGGAATTACTGGGCGTAAAGGGTGCGTA710.40382209077465586No Hit
TACGGAGGATCCGAGCGTTATCCGGCTTTATTGGGTTTAAAGGGAGCGTA580.3298828347173245No Hit
TACGAAGGGGGCGAGCGTTGTTCGGAATTACTGGGCGTAAAGGGTGTGTA580.3298828347173245No Hit
TACGTAGGGGGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGTGCGTA570.32419519963599136No Hit
TACGGAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGTGCGTA560.3185075645546582No Hit
TACGGAGGATCCAAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTA560.3185075645546582No Hit
TACGTAGGTGGCAAGCGTTGTCCGGAATTACTGGGTGTAAAGGGAGCGTA550.312819929473325No Hit
TACGGAGGATCCGAGCGTTATCCGGATTTCTTGGGTTTAAAGGGAGCGTA500.28438175406665905No Hit
ACGTAGGTCACAAGCGTTGTCCGGAATTACTGGGTGTAAAGGGAGCGCAG490.2786941189853259No Hit
TACGTAGGGAGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGCGCGCA490.2786941189853259No Hit
TACGTAGGTGGCGAGCGTTATCCGGAATTATTGGGCGTAAAGAGGGAGCA480.27300648390399274No Hit
TACGTAGGTGGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGCGTGTA460.26163121374132636No Hit
TACGGAGGATGCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGCA430.2445683084973268No Hit
TACGTAGGGAGCGAGCGTTGTCCGGAATTACTGGGTGTAAAGGGAGCGTA410.23319303833466043No Hit
TACGTATGGAGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGAGCGTA390.22181776817199408No Hit
AACGTAGGTCACAAGCGTTGTCCGGAATTACTGGGTGCAAAGGGAGCGCA380.2161301330906609No Hit
AACGTAGGTCACAAGCGTTGTCCGGACTTACTGGGTGTAAAGGGAGCGCA330.187691957683995No Hit
TACGTAGGGAGCGAGCGTTGTCCGGATTTACTGGGTGTAAAGGGTGCGTA330.187691957683995No Hit
TACGTAGGGGGCAAGCGTTATCCGGCTTTACTGGGTGTAAAGGGAGCGTA320.1820043226026618No Hit
TACGTAGGTCACAAGCGTTGTCCGGAATTACTGGGTGTAAAGGGAGCGCA320.1820043226026618No Hit
TACGGCGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTA310.17631668752132862No Hit
AACGTAGGTCACAAGCGTTGTCCGGAATTACTGGGTGTAAAGGGAGCGTA270.1535661471959959No Hit
AACGTAGGTAACAAGCGTTGTCCGGAATTACTGGGTGTAAAGGGAGCGCA270.1535661471959959No Hit
TACGTAGGGAGCAAGCGTTATCCGGATTTATTGGGTGTAAAGGGTGCGTA260.14787851211466274No Hit
AACGTAGGTCACAAGCGTTGTCCGGCATTACTGGGTGTAAAGGGAGCGCA250.14219087703332953No Hit
AACGGAGGTCACAAGCGTTGTCCGGAATTACTGGGTGTAAAGGGAGCGCA220.12512797178933No Hit
AACGTAGGTCACAAGCGTTGTCCGGAATTACTGGGTGTAAAGGGTGCGTA210.11944033670799681No Hit
TACGTAGGGGGCGAGCGTTATCCGGATTCATTGGGCGTAAAGCGCGCGTA200.11375270162666364No Hit
ACGTAGGGGGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGAGCGTAG200.11375270162666364No Hit
TACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGCACGCA190.10806506654533045No Hit
TACGGAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTC190.10806506654533045No Hit
TACGTAGGTGGCGAGCGTTGTTCGGATTTATTGGGCGTAAAGGGTCTGTA190.10806506654533045No Hit
TACGTAGGGGGCAAGCGTTATCCGGAATTACTGGGTGTAAAGGGAGCGTA190.10806506654533045No Hit
AACGTAGGTCACAAGCGCTGTCCGGAATTACTGGGTGTAAAGGGAGCGCA180.10237743146399728No Hit
ACCGTAGGTCACAAGCGTTGTCCGGAATTACTGGGTGTAAAGGGAGCGCA180.10237743146399728No Hit
TACGTAGGGGGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGAGTGTA180.10237743146399728No Hit
TACGTATGGGGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGAGCGTA180.10237743146399728No Hit
TACGGAAGATGCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGTGCGTA180.10237743146399728No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAGGGA750.0214.000024
TAGGGAG750.0214.000025
CAGCTCT100.00219472214.07
GAGGTCT100.00219472214.05
GAGGTCA100.00219472214.05
GTATGGA450.0214.04
AACGTAG3750.0214.01
GAGCGAG100.00219472214.09
AGCTCTA100.00219472214.08
CGGAGGT152.5631613E-5214.03
TACAGAG100.00219472214.01
AGGATTC100.00219472214.06
AGGATCC3500.0214.06
AGAGGTC100.00219472214.04
GGTCTCA100.00219472214.07
TACGGCG152.5631613E-5214.01
CACCGGC100.00219472214.01
ACGTATG1000.0214.02
TAGCTCA100.00219472214.05
GGATCCG3600.0214.07
Produced by FastQC (version 0.11.5)