FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005561816

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005561816
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences29026
Sequences flagged as poor quality0
Sequence length219
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTGTTTGCTACCCACACTTTCGAGCCTCAGCGTCAGTTGGTGCCCAGTA24298.368359401915525No Hit
CCTGTTTGATACCCACACTTTCGAGCCTCAATGTCAGTTGCAGCTTAGCA14514.998966443877903No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAACGTCAGATATAGTTTGGTA12804.409839454282367No Hit
CCTGTTCGATACCCACGCTTTCGTGCCTCAGCGTCTGTCGCACCCTAGCA11974.123888927168745No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTTACAGTCCAGTA9113.138565424102529No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTTACCGTCCAGTA8873.055880934334734No Hit
CCTGTTTGCTCCCCACACTTTCGCGCCTCAGCGTCAGTTACAGTCCAGTT8823.038654998966444No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTAATCGTCCAGTA8552.945634947977675No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTGAGCGTCAGTTATGTCCCAGGA7292.5115413766967545No Hit
CCTGTTTGATACCCACACTTTCGAGCATCAGCGTCAGTTACAGTCCAGCA6902.3771790808240887No Hit
CCTGTTTGCTACCCATGCTTTCGAGCCTCAGCGTCAGTTGGTGCCCAGTA6852.359953145455798No Hit
CCTGTTCGATACCCGCGCTTTCGAGCCTCAGCGTCAGTTGCGCTCCGGTA6842.35650795838214No Hit
CCTGTTTGATACCCACACTTTCGAGCATGAACGTCAGTTACAGTTTAGCA6472.2290360366567903No Hit
CCTGTTTGATACCCACACTTTCGAGCATCAGCGTCAGTTACACTCCAGTG6402.2049197271411836No Hit
CCTGTTCGCTCCCCACGCTTTCGCTCCTCAGCGTCAGTAACGGCCCAGAG6392.2014745400675255No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTCATCGTCCAGTA5902.032660373458279No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTTATCGTCCAGTA5801.9982085027216978No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTAAAAGCCCAGCA5151.7742713429339214No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAGTGTCAGTAGCAGTCCAGTA4421.52277268655688No Hit
CCTGTTTGCTCCCCACACTTTCGAGCCTCAGCGTCAGTTAGAGCCCAGCA3201.1024598635705918No Hit
CCTGTTTGATACCCACACTTTCGAGCATCAGCGTCAGTTACGCTCCAGCA2840.9784331289189003No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTTAATGTCCAGCA2740.9439812581823193No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCACCGTCAGTTGTCGTCCAGCA2730.9405360711086611No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGTGTCAGTTACAGTCCAGTA2630.9060842003720803No Hit
CCTGTTTGATCCCCACGCTTTCGTGCATCAGCGTCAGTAATGGCTTGGCA2620.9026390132984221No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAGTGTCAGTAACAGTCCAGCA2540.8750775167091573No Hit
CCTGTTTGCTACCCACGCTTTCGTGCCTCAGCGTCAGTTAAAGCCCAGCA2500.8612967684145248No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTATCGTCCAGTA2490.8578515813408669No Hit
CCTGTTCGCTCCCCACGCTTTCGCTCCTCAGCGTCAGTGACGGCCCAGAG2480.8544063942672088No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTTGTCGTCCAGCA2460.8475160201198925No Hit
CCTGTTTGCTCCCCACGCTTTCGCACCTCAGCGTCAATACCGGTCCAGGT2190.7544959691311238No Hit
CCTGTTTGATACCCACGCTTTCGTGCTTCAGTGTCAGTTATGGCTTGGCA2090.7200440983945429No Hit
CCTGTTTGATACCCACACTTTCGAGCATCAGTGTCAGTTGCAGTCCAGTG2020.6959277888789361No Hit
CCTGTTTGCTCCCCATGCTTTCGTCTTTCAGAGTCAGTTGCCGGCCAGTA2000.68903741473162No Hit
CCTGTTTGATCCCCATGCTTTCGTGCCTCAGCGTCAGTATATGGCCAGAT1920.6614759181423551No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTTTTCGTCCAGAA1880.6476951698477227No Hit
CCTGTTTGCTACCCACGCTTTCGCACCTCAGCGTCAGTTACCGTCCAGGT1790.6166884861847999No Hit
CCCTTTCGCTCCCCTGGCCTTCGTGCCTCAGCGTCAGTTAATGTCCAGGA1670.5753462413009026No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAACGTCAGCTATAGTTTGGTA1670.5753462413009026No Hit
CCTGTTTGCTCCCCACACTTTCGCGCCTCACCGTCAGTTATCGTCCAGCA1570.5408943705643217No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAGTGTCAGTTTCAGTCCAGTA1530.5271136222696893No Hit
CCTGTTTGCTCCCCACACTTTCGCGCCTCACCGTCAGTTGCTGTCCAGCA1520.5236684351960311No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTTACTGTCCAGTA1440.49610693860676636No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGTGTCAGTTACAGTCCAGTG1440.49610693860676636No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTCATCGTCCAGCA1420.48921656445945017No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGTGTCAGTTACAGTCCAGCA1400.482326190312134No Hit
CCTGTTTGCTCCCCACACTTTCGTGCCTCAACGTCAGTTTCAGTCCAGTA1320.4547646937228691No Hit
CCTGTTTGATCCCCACGCTTTCGTGCTTCAGTGTCAGTTATGGTTTAGTA1300.4478743195755529No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTCATCGTCCAGTA1260.4340935712809206No Hit
CCTGTTTGATACCCGCACTTTCGAGCCTCAGCGTCAGTGCTGCCCCGGCA1180.4065320746916558No Hit
CCTGTTCGCTCCCCACGCTTTCGCGCCTCACCGTCAGTTACCGTCCAGTA1150.39619651347068147No Hit
CCTGTTCGCTACCCACGCTCTCGTGCATCAGCGTCAGTTACAGTCTGGTA1120.3858609522497071No Hit
CCTGTTTGCTCCCCACACTTTCGTGCCTCAACGTCAGTTACTGTCCAGAA1080.3720802039550748No Hit
CCTATTTGCTCCCCACGCTTTCGTGCCTGAACGTCAGTAATGGCCCAGCA1070.3686350168814167No Hit
CCTGTTTGCTACCTACGCTTTCGTGCCTCAGCGTCAATGCTAGACCAGTA990.3410735202921519No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTGAACGTCAGTTACTGTCCAGCA870.29973127540825467No Hit
CCTGTTCGCTACCCACGCTCTCGCGCATCAGCGTCAGATCGAGCCTGGGA860.2962860883345966No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTACCGTCCAGTA830.2859505271136223No Hit
CCTGTTTGATACCCACACTTTCGAGCATCAGCGTCAGTTACAATCCAGTA800.27561496589264795No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAGCGTCAGTTACAGTCCAGAA790.27216977881898985No Hit
CCTGTTTGATACCCACACTTTCGAGCATCAGCGTCAGTTACAGTCCAGTA780.26872459174533175No Hit
CCTGTTTGATCCCCATGCTTTCGTGCCTCAGCGTCAATCAATGCCCAGAT780.26872459174533175No Hit
CCTGTTCGATACCCACGCTTTCGTGCTTCAGCGTCAGTTATAGCCCAGTA730.2514986563770413No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTGGTGCCCAGTA730.2514986563770413No Hit
CCTGTTTGATACCCACACTTTCGAGCATCAGTGTCAGTGACAGTCTAGTG720.24805346930338318No Hit
CCTGTTTGATACCCACGCTTTCGTGCTTCAGTGTCAGTTATGGCTTGGGA690.2377179080824089No Hit
CCTATTCGCTCCCCACGCTTTCGTGTCTCAGCGTCAGTAATGGCCCAGCA690.2377179080824089No Hit
CCTGTTTGATCCCCATGCTTTCGTGCCTCAGCGTCAGTTGACAGCCAGAT690.2377179080824089No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAGTGTCAGTTACAGTCCAGTA680.2342727210087508No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTAAAGGCCCAGCA670.2308275339350927No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTGAACGTCAGTTACTGTCCAGTA660.22738234686143455No Hit
CCTATTTGCTCCCCACGCTTTCGTGCCTCAGCGTCAGTAACGGCCCAGCA640.22049197271411836No Hit
CCTGTTTGATACCCACGCTTTCGTGCTTCAGTGTCAGTTATGGCTTTGCA630.2170467856404603No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCATTGTCAGTCCAGTA520.17914972783022118No Hit
CCTGTTCGCTACCCACGCTCTCGTGCATCAGCGTCAGTTATGGTCTGGTA510.17570454075656308No Hit
CCTGTTTGATACCCACACTTTCGAGCATCAGTGTCAGTAACAGTCTAGTG500.172259353682905No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTACAGTCCAGTA500.172259353682905No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTTTCAGTCCAGAA490.16881416660924686No Hit
CCTGTTTGCTCCCCACGCTTTCGTGTCTCAGCGTCAGTTGTGGTCCAGTA460.15847860538827258No Hit
CCCTTTCGCTCCCCACGCTTTCGAGCATGAGCGTCAGTTTCTGTCCAGTA420.1446978570936402No Hit
CCTATTTGCTCCCCACGCTTTCGTGCCTCAGCGTCAGTTACAGCCCAGCA400.13780748294632397No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTTCAGTCCAGAA390.13436229587266588No Hit
CCTGTTCGATACCCACGCTTTCGTGCTTCAGCGTCTGTTTCACCCCAGTA380.13091710879900778No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTTGCCGTCCAGTA350.1205815475780335No Hit
CCTGTTTGCTCCCCACACTTTCGAGCCTCAGCGTCAGTTAAAGCCCAGTT340.1171363605043754No Hit
CCTGTTTGCTCCCCACGCTTTCGCTCCTCAGCGTCAGTTAATGTCCAGCA340.1171363605043754No Hit
CCTGTTTGCTCCCCATGCTTTCGTCTTTCAGAGTCAGTTTCCGTCCAGCA330.11369117343071727No Hit
CCTGTTCGCTACCCACGCTCTCGTGCATCAGCGTCAGTTTCAGTCTGGTA320.11024598635705918No Hit
CCTGTTCGCTCCCCACGCTTTCGTGCCTCAGCGTCAGTAATAGCTTGGAA320.11024598635705918No Hit
CCTGTTTGCTCCCCACGCTTTCGTGTCTCAGCGTCAGTTGTCGTCCAGTA320.11024598635705918No Hit
CCTGTTTGCTCCCCACACTTTCGCGCCTCAGCGTCAGTTACAGTCCAGTA320.11024598635705918No Hit
CCTGTTTGATACCCACACTTTCGAGCCTCAGCGTCAGTTGGTGCCCAGTA310.1068007992834011No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCTGTTT24250.0213.000021
CCTGTTC3650.0213.000021
TGCTACC4100.0213.000027
TTTGCTA4100.0213.000025
TTGCTAC4100.0213.000026
ATCCCCA1000.0213.09
CCTATTC100.0022332042213.01
TGATCCC1000.0213.07
GCTACCT100.0022332042213.08
TCGCTAC450.0213.06
GATCCCC1000.0213.08
ATTCGCT100.0022332042213.04
TATTCGC100.0022332042213.03
TATTTGC400.0213.03
TTGATCC1000.0213.06
ATTTGCT400.0213.04
CTACCTA100.0022332042213.09
CTATTTG400.0213.02
CTATTCG100.0022332042213.02
TTTGATC1000.0213.05
Produced by FastQC (version 0.11.5)