FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005561857

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005561857
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28409
Sequences flagged as poor quality0
Sequence length220
%GC51

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TACGGAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTA320511.281636101235524No Hit
TACGGAGGATGCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGTGCGTA287210.10947235031152No Hit
AACGTAGGTCACAAGCGTTGTCCGGAATTACTGGGTGTAAAGGGAGCGCA28129.898271674469358No Hit
TACGTAGGGGGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGAGCGTA23258.184026188883804No Hit
AACGTAGGGTGCAAGCGTTGTCCGGAATTACTGGGTGTAAAGGGAGCGCA20687.279383294026541No Hit
TACGGAGGATTCAAGCGTTATCCGGATTTATTGGGTTTAAAGGGTGCGTA15135.3257770424865365No Hit
TACGTATGGTGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGAGCGCA10763.7875321201027843No Hit
TACGTAGGGAGCAAGCGTTGTCCGGATTTACTGGGTGTAAAGGGTGCGTA10363.6467316695413428No Hit
TACGTAGGTGGCAAGCGTTGTCCGGATTTACTGGGTGTAAAGGGCGTGTA8102.8512091238691966No Hit
TACGTAGGGGGCAAGCGTTATCCGGAATTATTGGGCGTAAAGGGTGCGTA7862.766728853532331No Hit
TACGTAGGGTGCAAGCGTTATCCGGAATTATTGGGCGTAAAGGGCTCGTA7012.4675278960892677No Hit
TACGGAGGATGCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGCA6812.3971276708085467No Hit
TACGTAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGTGCGCA5511.9395262064838608No Hit
TACGTAGGTGGCAAGCGTTGTCCGGATTTACTGGGTGTAAAGGGCGTGCA4861.710725474321518No Hit
TACGTAGGGGGCAAGCGTTGTCCGGAATGACTGGGCGTAAAGGGAGTGTA3621.2742440775810482No Hit
TACGTAGGGGGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGAGCGCA3131.101763525643282No Hit
TACGTATGGTGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGAGCGTA2800.9856031539300927No Hit
TACGTAGGTGGCGAGCGTTATCCGGATTTACTGGGTGTAAAGGGCGCGTA2710.9539230525537682No Hit
TACGGAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGTGCGTA2170.7638424442958217No Hit
TACGGAAGATGCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGTGCGTA1710.6019219261501636No Hit
TACGTAGGGGGCAAGCGTTATCCGGAATTACTGGGTGTAAAGGGTGCGTA1600.5632018022457672No Hit
TACGTATGGTGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGTGCGTA1550.545601745925587No Hit
TACGGAGGATGCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTA1450.5104016332852265No Hit
TACGTAGGTGGCGAGCGTTATCCGGAATTACTGGGTGTAAAGGGTGTGTA1340.4716815093808301No Hit
TACGTAGGTGGCAAGCGTTGTCCGGAATTATTGGGCGTAAAGCGCGCGCA1330.46816149811679403No Hit
TACGTAGGATGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGAGCGCA1290.4540814530606498No Hit
TACGGAAGATGCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTA1110.39072125030800103No Hit
TACGTAGGTGGCGAGCGTTATCCGGATTTACTGGGTGTAAAGGGTGTGTA1070.3766412052518568No Hit
TACGTATGGAGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGAGTGTA1060.3731211939878208No Hit
TACGGAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGCA1040.36608117145974867No Hit
TACGTATGGGGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGAGCGTA1030.36256116019571266No Hit
TACGTAGGTGGCAAGCGTTGTCCGGAATTACTGGGTGTAAAGGGAGCGCA940.33088105881938823No Hit
TACGTAGGTGGCAAGCGTTGTCCGGAATTACTGGGTGTAAAGGGAGCGTA920.32384103629131616No Hit
TACGGAGACTACGAGCGTTACTCGGATTCACTGGGCGTAAAGGGAGCGCA920.32384103629131616No Hit
TACGTAGGGGGCTAGCGTTATCCGGATTTACTGGGCGTAAAGGGTGCGTA900.31680101376324404No Hit
TACGTAGGGGGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGTGCGTA840.2956809461790278No Hit
TACGTAGGTGGCAAGCGTTGTCCGGAATTACTGGGTGTAAAGGGAGTGTA830.29216093491499173No Hit
TACGTAGGGCGCAAGCGTTGTCCGGATTTACTGGGTGTAAAGGGTGCGTA780.2745608785948115No Hit
TACGTAGGGTGCAAGCGTTGTCCGGAATTACTGGGTGTAAAGGGAGCGCA650.22880073216234292No Hit
TACAGAGGTCTCAAGCGTTGTTCGGAATCACTGGGCGTAAAGCGTGCGTA630.22176070963427086No Hit
TACGGAGGATTCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTA620.2182406983702348No Hit
TACGTAGGTGGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGCGCGTA560.19712063078601855No Hit
TACGTAGGGTGCGAGCGTTAATCGGAATTACTGGGCGTAAAGCGTGCGCA540.19008060825794643No Hit
TACGTAGGGAGCGAGCGTTGTCCGGATTTACTGGGTGTAAAGGGTGCGTA540.19008060825794643No Hit
TACGTATGGAGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGTGCGTA530.1865605969939104No Hit
TACGTATGGAGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGAGCGTA510.1795205744658383No Hit
TACGTAGGGAGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGCGCGCA470.16544052940969411No Hit
TACGGAGGGTGCAAGCGTTAATCGGAATCACTGGGCGTAAAGCGCACGTA430.15136048435354993No Hit
TACGTAGGTGGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGCGTGTA410.14432046182547784No Hit
TACGTAGGGAGCGAGCGTTGTCCGGAATGACTGGGTGTAAAGGGAGCGTA380.1337604280333697No Hit
TACGTAGGGGGCAAGCGTTATCCGGAATTATTGGGCGTAAAGAGTACGTA330.11616037171318949No Hit
TACGTAGGGTGCAAGCGTTACTCGGAATTACTGGGCGTAAAGCGTGCGCA320.11264036044915343No Hit
TACGTAGGGGGCGAGCGTTGTCCGGAATGACTGGGCGTAAAGGGCGTGTA310.1091203491851174No Hit
TACGTAGGTGGCAAGCGTTGTCCGGATTTACTGGGTGTAAAGGGTGCGTA300.10560033792108134No Hit
TACGTAGGTCACAAGCGTTGTCCGGAATTACTGGGTGTAAAGGGAGCGCA290.1020803266570453No Hit
TACGTAGGTGGCAAGCGTTATCCGGAATGACTGGGCGTAAAGGGTGCGTA290.1020803266570453No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATGCGA4700.0214.000028
ATGCGAG4700.0214.000029
CGTAGGG9150.0214.000023
TGGCGAG650.0214.000029
GTATGGA253.5342964E-9214.04
GAGCGAG203.0137016E-7214.09
TAGGATG253.5342964E-9214.05
GTCACAA4000.0214.08
GAGGATT2250.0214.05
GAGGATC3750.0214.05
AGGATTC2250.0214.06
AGGATCC3750.0214.06
TACGGAA600.0214.01
GAAGATG600.0214.05
CGTAGGA253.5342964E-9214.03
GGTCACA4000.0214.07
ATGGAGC253.5342964E-9214.06
TACGTAT1600.0214.01
GGGGGCG203.0137016E-7214.07
AAGATGC600.0214.06
Produced by FastQC (version 0.11.5)