FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005561864

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005561864
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences38451
Sequences flagged as poor quality0
Sequence length219
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTGTTTGCTACCCACACTTTCGAGCCTCAGCGTCAGTTGGTGCCCAGTA1066227.728797690567212No Hit
CCTGTTTGATCCCCACGCTTTCGTGCATCAGCGTCAGTCATGGCTTGGCA438411.401524017580817No Hit
CCTGTTTGATACCCACACTTTCGAGCATCAGCGTCAGTTACAGTCCAGTA426211.084237080960182No Hit
CCTGTTTGATACCCACACTTTCGAGCCTCAATGTCAGTTGCAGCTTAGCA31958.309276741827262No Hit
CCTGTTTGATACCCACACTTTCGAGCATCAGTGTCAGTTGCAGTCCAGTG14883.8698603417336352No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTAAAGCCCAGTA14803.849054640971626No Hit
CCTGTTTGATACCCACACTTTCGAGCATCAGCGTCAGTTACACTCCAGTG10142.637122571584614No Hit
CCTGTTTGATACCCACACTTTCGAGCATCAGCGTCAGTTACAATCCAGTA8832.2964292216067204No Hit
CCTGTTTGATACCCACACTTTCGAGCATCAGTGTCAGTTGCAGTCTAGTG8052.0935736391771345No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTTACCGTCCAGTA7031.8283009544615223No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAACGTCAGATATAGTTTGGTA5641.4668019037216198No Hit
CCTGTTTGATCCCCACGCTTTCGTGCTTCAGTGTCAGTTATAGTTTAGTA5581.451197628150113No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTTACTGTCCAGTA4331.1261085537437259No Hit
CCTGTTTGATCCCCATGCTTTCGTGCCTCAGCGTCAATCAATGCCCAGAT4321.1235078411484747No Hit
CCTGTTTGATACCCGCACTTTCGAGCCTCAGCGTCAGTGCTGCCCCGGCA3490.9076486957426334No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTTATCGTCCAGTA3220.8374294556708538No Hit
CCTGTTTGCTCCCCACGCTTTCGCACCTGAGCGTCAGTCTTCGTCCAGGG2650.6891888377415412No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCATGAGCGTCAGTCGCATCCCAGGG2420.629372448050766No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTTACTGTCCAGCA2150.5591532079789863No Hit
CCTGTTCGATACCCACGCTTTCGTGCTTCAGCGTCAGTTATAGTATGGTA1920.4993368182882109No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCACCGTCAGTTGTCGTCCAGCA1810.47072897974044886No Hit
CCGGTTCGCGCCCCTAGCTTTCGTCCCTCACCGTCAGAATCGTTCCAGTC1720.44732256638318896No Hit
CCTGTTTGATACCCACACTTTCGAGCATCAGCGTCAGTTACAGTCCAGCA1620.42131544043067803No Hit
CCTGTTCGCTCCCCACGCTTTCGCTCCTCAGCGTCAGTAACGGCCCAGAG1530.3979090270734181No Hit
CCTGTTTGCTACCCACGCTTTCGTGCCTCAGCGTCAGTTAAAGCCCAGCA1480.38490546409716264No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCATTTACAGTCCAGTA1390.36149905073990274No Hit
CCTGTTTGATCCCCACGCTTTCGTGCATCAGCGTCAGTCATGGCTTTGCA1360.35369691295414946No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAGTGTCAGTTACAGTCCAGTG1340.34849548776364725No Hit
CCTGTTCGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTACAGACCAGAG1220.31728693662063406No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTGAGCGTCAGTTATGTCCCAGGA970.2522691217393566No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTGGTGCCCAGTA960.24966840914410546No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAGTGTCAGTTACAGTCCAGAA930.2418662713583522No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTTACAGTCCAGTA850.2210605705963434No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTTGCTGTCCAGCA800.20805700762008789No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTCACTGTCCAGTA770.2002548698343346No Hit
CCTATTTGCTCCCCACGCTTTCGTGCTTCAGTGTCAGAATCCAGACCAGA770.2002548698343346No Hit
CCTGTTTGCTACCCATGCTTTCGAGCCTCAGCGTCAGTTGGTGCCCAGTA760.1976541572390835No Hit
CCTGTTCGCTACCCACGCTCTCGCGCATCAGCGTCAGATCGAGCCTGGGA720.18725130685807911No Hit
CCTGTTTGATCCCCACGCTTTCGTGCATCAGCGTCAGTCATGGCTTGGCC630.1638448935008192No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTTGTCGTCCAGCA600.15604275571506593No Hit
CCTGTTTGCTCCCCACGCTTTCGCACCTGAGCGTCAGTCTTTGTCCAGGG600.15604275571506593No Hit
CCTGTTTGCTACCCACGCTTTCGCACCTCAGCGTCAGTTACCGTCCAGGT590.15344204311981482No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTTGCCGTCCAGTA570.14824061792931262No Hit
CTGTTTGCTACCCACACTTTCGAGCCTCAGCGTCAGTTGGTGCCCAGTAG560.14563990533406151No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTTACTGTCCAGCA560.14563990533406151No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTGACTGTCCAGTA540.14043848014355934No Hit
CCTGTTTGATACCCACACTTTCGAGCCTCAGCGTCAGTTGGTGCCCAGTA530.13783776754830823No Hit
CCTGTTTGATCCCCATGCTTTCGTGCCTCAGCGTCACTCAATGCCCAGAT530.13783776754830823No Hit
CCTGTTTGCTCCCCACGCTTTCGCTCCTCAGCGTCAGTTAATGTCCAGCA520.13523705495305713No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTCATCGTCCAGCA520.13523705495305713No Hit
CCTATTTGCTCCCCACGCTTTCGTGACTGAGCGTCAGTTACGGACTGGTA510.13263634235780603No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAACGTCAGCTATAGTTTGGTA510.13263634235780603No Hit
CCTGTTTGATCCCCACGCTTTCGTGCATCAGCGTCAGTCATGGCTTAGCA500.13003562976255492No Hit
CCTGTTTGCTCCCCACACTTTCGAGCCTCAGCGTCAGTAAAAGCCCAGTT480.12483420457205273No Hit
CCTGTTCGCTCCCCACGCTTTCGCGCCTCACCGTCAGTTACCGTCCAGTA470.12223349197680164No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTTAATGTCCAGCA440.11443135419104834No Hit
CCTGTTTGATCCCCACGCTTTCGTGCTTCAGTGTCAGTTATGGTTTAGTA420.10922992900054614No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCATTGTCAGTCCAGTA410.10662921640529505No Hit
CCTGTTCGCTCCCCCAGCTTTCGCGCCTCAGCGTCGGTCTCGGCCCAGAG390.10142779121479284No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGATACC253.6598067E-9213.000027
TCGATAC253.6598067E-9213.000026
TTCGATA253.6598067E-9213.000025
TGTTCGA253.6598067E-9213.000023
ATACCCG350.0213.000029
GTTCGAT253.6598067E-9213.000024
CCTATTT304.3655746E-11213.01
CCTGTTC800.0213.01
CGCCCCT203.0990668E-7213.09
TGTTCGC550.0213.03
GGTTCGC203.0990668E-7213.03
GCGCCCC203.0990668E-7213.08
TATTTGC304.3655746E-11213.03
ATTTGCT304.3655746E-11213.04
TCCTGTT100.0022360317213.01
CTGTTCG800.0213.02
CGGTTCG203.0990668E-7213.02
CCGGTTC203.0990668E-7213.01
CTATTTG304.3655746E-11213.02
GTTCGCT550.0213.04
Produced by FastQC (version 0.11.5)