FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005562019

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005562019
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25446
Sequences flagged as poor quality0
Sequence length220
%GC50

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TACGGAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTA23869.376719327202704No Hit
AACGTAGGGTGCAAGCGTTGTCCGGAATTACTGGGTGTAAAGGGAGCGCA18327.199559852236108No Hit
TACGTAGGGGGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGAGCGTA13295.222824805470408No Hit
TACGTAGGTGGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGCGCGTA11544.535093924388902No Hit
AACGTAGGTCACAAGCGTTGTCCGGAATTACTGGGTGTAAAGGGAGCGCA8163.206790851214336No Hit
TACGTAGGGAGCGAGCGTTGTCCGGAATTACTGGGTGTAAAGGGAGCGTA7262.85310068380099No Hit
TACGTAGGTGGCAAGCGTTGTCCGGATTTACTGGGTGTAAAGGGCGTGTA7062.774502868820247No Hit
TACGGAGGATGCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGCA6712.636956692603946No Hit
TACGTAGGGGGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGAGCGCA6022.3657942309203803No Hit
TACGTATGGTGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGAGCGCA5112.008174172757997No Hit
TACGTAGGGGGCGAGCGTTGTCCGGAATGACTGGGCGTAAAGGGAGTGTA5001.9649453745185883No Hit
TACGTATGGTGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGTGCGTA4681.8391888705493988No Hit
TACGGAGGATGCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGTGCGTA3771.4815688123870157No Hit
TACGTAGGGTGCGAGCGTTAATCGGAATTACTGGGCGTAAAGGGTGCGCA3741.469779140139904No Hit
TACGTAGGGAGCAAGCGTTGTCCGGATTTACTGGGTGTAAAGGGTGCGTA3571.402970997406272No Hit
TACGGAGGATCCAAGCGTTATCCGGATTTATTGGGTTTAAAGGGTGCGTA3391.332232963923603No Hit
TACGTAGGTGGCAAGCGTTGTCCGGATTTACTGGGTGTAAAGGGCGTGCA3381.3283030731745658No Hit
TACGAAGGGGGCGAGCGTTGTTCGGAATTACTGGGCGTAAAGGGTGTGTA3341.3125835101784171No Hit
TACGTAGGGAGCAAGCGTTATCCGGATTTATTGGGTGTAAAGGGTGCGTA2821.1082291912284838No Hit
TACAGAGGTCTCAAGCGTTGTTCGGAATCACTGGGCGTAAAGCGTGCGTA2821.1082291912284838No Hit
TACATAGGGGGCGAGCGTTATCCGGATTTATTGGGCGTAAAGCGTGCGTA2771.088579737483298No Hit
TACGGAGGATTCAAGCGTTATCCGGATTTATTGGGTTTAAAGGGTGCGTA2390.9392438890198852No Hit
TACGGAGGGTGCAAGCGTTGTCCGGAATCATTGGGCGTAAAGCGTTCGTA2360.9274542167727736No Hit
TACGTATGGAGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGAGTGTA2170.8527862925410674No Hit
TACGGAGGATTCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTA2120.8331368387958815No Hit
TACGTAGGGGGCAAGCGTTATCCGGAATTACTGGGTGTAAAGGGTGCGTA1870.734889570069952No Hit
TACGTAGGTGGCAAGCGTTATCCGGAATTACTGGGTGTAAAGGGAGCGCA1840.7230998978228406No Hit
TACGTAGGGAGCAAGCGTTATCCGGAATTACTGGGTGTAAAGGGAGTGTA1760.6916607718305431No Hit
TACGTAGGGAGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGCGCGCA1730.6798710995834315No Hit
TACGTAGGTGGCGAGCGTTATCCGGATTTACTGGGTGTAAAGGGTGTGTA1690.6641515365872829No Hit
TACGTAGGGAGCGAGCGTTATCCGGATTTATTGGGTGTAAAGGGTGCGTA1690.6641515365872829No Hit
TACGGAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGTGCGTA1680.6602216458382457No Hit
TACGTAGGGGGCGAGCGTTGTCCGGAATTACTGGGCGTAAAGGGAGCGTA1500.5894836123555766No Hit
TACGTAGGATGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGAGCGCA1490.5855537216065393No Hit
TACGTAGGTGGCAAGCGTTGTCCGGAATTACTGGGTGTAAAGGGAGCGCA1450.5698341586103907No Hit
TACGGAGGATGCAAGCGTTATCCGGATTTATTGGGTTTAAAGGGTGCGTA1400.5501847048652048No Hit
TACGTAGGTGGCGAGCGTTATCCGGAATTATTGGGCGTAAAGAGGGAGCA1320.5187455788729073No Hit
TACGTAGGTGGCGAGCGTTGTCCGGAATGACTGGGCGTAAAGGGAGCGTA1250.4912363436296471No Hit
TACGGAGGGTGCAAGCGTTAATCGGAATCACTGGGCGTAAAGCGCACGTA1240.4873064528806099No Hit
TACGTATGGAGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGTGCGTA1230.48337656213157276No Hit
TACGTATGGTGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGAGCGTA1200.47158688988446124No Hit
TACGTAGGTTGCAAGCGTTGTCCGGATTTACTGGGTGTAAAGGGCGTGTA1200.47158688988446124No Hit
TACGTAGGTGGCAAGCGTTGTCCGGAATTACTGGGTGTAAAGGGAGCGTA1190.467656999135424No Hit
TACGGAGGATGCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTA1040.40870863789986644No Hit
TACGTAGGTGGCGAGCGTTATCCGGATTTACTGGGTGTAAAGGGCGCGTA1020.400848856401792No Hit
TACGTAGGGTGCAAGCGTTATCCGGAATTATTGGGCGTAAAGGGCTCGTA1020.400848856401792No Hit
TACGTAGGTGGCGAGCGTTATCCGGATTTATTGGGCGTAAAGGGTGCGTA1000.3929890749037177No Hit
TACGTAGGTGGCAAGCGTTGTCCGGATTTACTGGGTGTAAAGGGCGCGTA830.32618093217008565No Hit
TACGTAGGGGGCAAGCGTTGTCCGGAATGACTGGGCGTAAAGGGTGTGTA760.29867169692682544No Hit
TACGGAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGCA730.2868820246797139No Hit
TACGTAGGGAGCAAGCGTTGTCCGGAATGACTGGGCGTAAAGGGCGCGTA690.2711624616835652No Hit
TACGTAGGTGGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGCGTGTA690.2711624616835652No Hit
TACGTAGGGAGCGAGCGTTGTCCGGATTTACTGGGTGTAAAGGGTGCGTA680.267232570934528No Hit
TACGTAGGGGGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGTGCGTA670.26330268018549086No Hit
TACGTATGGAGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGAGCGTA620.24365322644030496No Hit
TACGTAGGTGGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGTGAGTA580.22793366344415625No Hit
TACGTAGGGAGCGAGCGTTGTCCGGAATTACTGGGTGTAAAGGGTGCGTA550.21614399119704472No Hit
TACGTAGGGAGCGAGCGTTGTCCGGAATTACTGGGTGTAAAGGGAGCGCA540.21221410044800754No Hit
TACGGAGACTACGAGCGTTACTCGGATTCACTGGGCGTAAAGGGAGCGCA540.21221410044800754No Hit
TACGTAGGGTGCAAGCGTTGTCCGGAATTACTGGGTGTAAAGGGAGCGCA520.20435431894993322No Hit
TACGTAGGTGGCGAGCGTTATCCGGATTTATTGGGCGTAAAGGGTGCGCA520.20435431894993322No Hit
TACGTAGGGGGCGAGCGTTATCCGGATTTATTGGGCGTAAAGCGTGCGTA490.19256464670282167No Hit
TACGTAGGTGGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGTGTGTA480.18863475595378448No Hit
TACGTAGGGGGCGAGCGTTGTCCGGAATGACTGGGCGTAAAGGGTGTGTA480.18863475595378448No Hit
TACGTAGGGGGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGCGCGTA440.17291519295763577No Hit
TACGTAGGTGGCGAGCGTTGTCCGGAATTATTGGGCGTAAAGAGCATGTA440.17291519295763577No Hit
TACGTAGGGAGCGAGCGTTGTCCGGAATGACTGGGTGTAAAGGGAGCGTA420.16505541145956143No Hit
TACGGAAGATGCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGTGCGTA410.16112552071052424No Hit
TACGGAGGATCCGAGCGTTATCCGGCTTTATTGGGTTTAAAGGGAGCGTA400.15719562996148706No Hit
AACGTAGGGTGCAAGCGTTGTCCGGAATTACTGGGTGTAAAGGGAGCGTA390.15326573921244988No Hit
TACGTAGGGGGCAAGCGTTATCCGGAATTATTGGGCGTAAAGAGTGCGTA390.15326573921244988No Hit
TACATAGGGGGCAAGCGTTATCCGGAATTATTGGGCGTAAAGCGTGCGTA380.14933584846341272No Hit
TACGTAGGATGCAAGCGTTATCCGGAATGACTGGGCGTAAAGGGTGCGTA380.14933584846341272No Hit
TACGTAGGGGGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGAGTGTA360.14147606696533838No Hit
TACGTAGGTAGCAAGCATTGTCCGGAATAACTGGGCGTAAAGGGAGCGTA350.1375461762163012No Hit
TACGTAGGTGGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGAGCGTA330.12968639471822682No Hit
TACGTAGGGGGCGAGCGTTGTCCGGAATTACTGGGCGTAAAGGGTGCGTA330.12968639471822682No Hit
TACGTAGGTGGCAAGCGTTGTCCGGAATTACTGGGTGTAAAGGGTGCGCA320.12575650396918966No Hit
TACGTATGGGGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGAGCGTA320.12575650396918966No Hit
TACGTAGGGGGCAAGCGTTGTCCGGAATGATTGGGCGTAAAGGGCGCGTA310.12182661322015248No Hit
TACGTAGGTGGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGAGCGCA290.11396683172207812No Hit
TACGTAGGGAGCGAGCGTTGTCCGGAATTACTGGGTGTAAAGGGTGAGTA290.11396683172207812No Hit
TACGTAGGGGGCGAGCGTTGTCCGGAATGACTGGGCGTAAAGGGCGTGTA290.11396683172207812No Hit
TACGGAGGATGCGAGCGTTATCCGGAATTATTGGGTTTAAAGGGTCCGCA280.11003694097304094No Hit
TACGTAGGTGGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGTGCGTA270.10610705022400377No Hit
TACGTAGGTGGCGAGCGTTATCCGGAATGATTGGGCGTAAAGGGTGCGTA260.10217715947496661No Hit
TACGGAGGATCCGAGCGTTATCCGGATTTCTTGGGTTTAAAGGGAGCGTA260.10217715947496661No Hit
TACGTAGGGGGCAAGCGTTATCCGGAATTATTGGGCGTAAAGAGTACGTA260.10217715947496661No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAGCTCT100.002200508214.07
GTATGGA700.0214.04
AACGTAG3000.0214.01
GAGCGAG1400.0214.09
AGCTCTA100.002200508214.08
GTCACAA900.0214.08
GAGGATT304.1836756E-11214.05
ACAGAGG253.5233825E-9214.02
TACAGAG253.5233825E-9214.01
AGGATTC304.1836756E-11214.06
ACGTCGG152.5767138E-5214.02
AGGATGC1700.0214.06
AGAGGTC253.5233825E-9214.04
CACCGGC100.002200508214.01
GATGCGA1250.0214.08
ACGTAGT100.002200508214.02
ATGCGAG1250.0214.09
TGGAGCA700.0214.07
ATGGAGC700.0214.06
TACGTCG152.5767138E-5214.01
Produced by FastQC (version 0.11.5)