FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005562190

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005562190
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences29882
Sequences flagged as poor quality0
Sequence length219
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTGTTTGATACCCGCACTTTCGAGCATCAACGTCAGTTACGGTCCAGCA24698.262499163375946No Hit
CCTGTTTGCTCCCCACGCTTTCGCACCTCAGCGTCAATACCGGTCCAGGT20636.903821698681481No Hit
CCTGTTTGATACCCACACTTTCGAGCATCAGCGTCAGTTACAGTCCAGCA16585.548490730205475No Hit
CCTGTTTGATACCCACACTTTCGAGCCTCAACGTCAGTCGCGGCTTAGCA16315.458135332307075No Hit
CCTGTTTGATCCCCACGCTTTCGTGCATCAGCGTCAGTAATGGCTTGGCA13774.6081252928184195No Hit
CCTGTTTGATACCCACACTTTCGAGCATCAGTGTCAGTTGCAGTCCAGTG13474.507730406264641No Hit
CCTGTTTGATACCCACACTTTCGAGCATCAACGTCAGTTACGCTCCAGCA12304.116190348704906No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCATGAGCGTCAGTCTTGGCCCAGGA10643.5606719764406667No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCATGAGCGTCAGTCGCATCCCAGGG10633.5573254802222074No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTATCGTCCAGTA8822.9516096646810785No Hit
CCTGTTTGATACCCACACTTTCGAGCCTCAATGTCAGTTGCAGCTTAGCA8252.7608593802289003No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTAAAAGCCCAGCA7362.463021216786025No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAACGTCAGATATAGTTTGGTA6952.325814871829195No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTTACCGTCCAGTA5911.9777792651094306No Hit
CCTGTTTGCTACCCACACTTTCGAGCCTCAGCGTCAGTTGGTGCCCAGTA5891.971086272672512No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTCACTGTCCAGTA5711.910849340740245No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAGTGTCAGTTATGGGCCAAAT5361.7937219730941705No Hit
CCTGTTTGATACCCACACTTTCGAGCATCAGCGTCAGTTACACTCCAGTG4051.3553309684760055No Hit
CCTGTTCGATACCCACGCTTTCGTGCCTCAGCGTCTGTCGCACCCTAGCA3831.2817080516699018No Hit
CCTATTTGCTCCCCACGCTTTCGGGACTGAGCGTCAGCTGCACCCCAGAC3321.1110367445284788No Hit
CCTGTTCGCTACCCACGCTTTCGTGCATCAGCGTCAGATATAGATTAGCA3161.0574928050331303No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTTACAGTCCAGTA3111.040760323940834No Hit
CCTGTTTGCTCCCCACACTTTCGCGCCTCAGCGTCAGTAACAGTCCAGTA2710.9069004752024631No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTTAATGTCCAGCA2600.8700890167994111No Hit
CCTGTTTGATCCCCACGCTTTCGTGCTTCAGTGTCAGTTATAGTTTAGTA2430.8131985810856033No Hit
CCTGTTTGATCCCCACGCTTTCGTGCTTCAGTGTCAGTTATGGTTTAGTA2140.7161501907502844No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTTACTGTCCAGTA2110.7061107020949067No Hit
CCTATTTGCTCCCTGTGCTTTCGTGACTGAGCGTCAGTATTGTGCTGGTA2110.7061107020949067No Hit
CCTGTTTGATCCCCACGCTTTCGTGCATCAGCGTCAGTCATGGCTTGGCA1750.5856368382303728No Hit
CCTGTTTGCTCCCCACGCTTTCGCACCTCAGCGTCACTACCGGTCCAGGT1710.5722508533565357No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTTACTGTCCAGCA1500.501974432768891No Hit
CCTGTTTGCTACCCATGCTTTCGAGCCTCAGCGTCAGTTAGTGCCCAGTA1500.501974432768891No Hit
CCCATTTGCTACCCCGGCTTTCGTTCCTCAGTGTCAATTACGGCCCAGTA1340.4484304932735426No Hit
CCTGTTTGATACCCACACTTTCGAGCATCAACGTCAGTTACGGTCCAGCA1250.41831202730740913No Hit
CCTGTTTGCTACCCACGCTTTCGCACCTCAGCGTCAGTTTTGTGCCAGGC1230.41161903487049056No Hit
CCTGTTCGCTACCCACGCTCTCGCGCATCAGCGTCAGATCGAGCCTGGGA1160.3881935613412757No Hit
CCTGTTTGCTCCCCACGCTTTCGCCCCTCAGCGTCAATACCGGTCCAGGT1100.36811458403052No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGTGTCAGTTACAGTCCAGTG1090.36476808781206077No Hit
CCTGTTTGCTACCCATGCTTTCGAGCCTCAGCGTCAGTTGGTGCCCAGCA1050.3513821029382237No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCATGAGCGTCAGTTTTGCCCCAGGG1010.3379961180643866No Hit
CCTGTTTGATCCCCACGCTTTCGTGCATCAGCGTCAGTAATGGCTTTGCA1000.33464962184592734No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTTGCTGTCCAGCA960.32126363697209026No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTTACTGTCCAGCA940.3145706445351717No Hit
CCTGTTTGCTACCCACACTTTCGTGCCTCAGCGTCAGTTAAAGCCCAGTA940.3145706445351717No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAGTGTCAGTAACAGTCCAGCA920.30787765209825313No Hit
CCTGTTTGATACCCACACTTTCGAGCATGAACGTCAGTTACGGCTTAGTG880.294491667224416No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAGTGTCAGTTTCAGTCCAGTA880.294491667224416No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAACGTCAGCTATAGTTTGGTA870.29114517100595677No Hit
CCTGTTTGATACCCACACTTTCGAGCATCAGCGTCAGTTACAGTCCAGTA830.2777591861321197No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTAATCGTCCAGTA790.26437320125828256No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGTGTCAGTTACAGTCCAGTA760.25433371260290477No Hit
CCTGTTTGATACCCACACTTTCGAGCATCAGTGTCAGTTGCAGTCTAGTG760.25433371260290477No Hit
CCCGTTCGCTACCCTGGCTTTCGCATCTCAGCGTCAGACACAGTCCAGAA730.24429422394752695No Hit
CCTGTTTGATACCCTCACTTTCGAGCATCAACGTCAGTTACGGTCCAGCA730.24429422394752695No Hit
CCTGTTTGCTCCCCACGCTTTCGCACCTGAGCGTCAGTATTTGCCCAGAA730.24429422394752695No Hit
CCTGTTTGCTACCCACGCTTTCGTGCCTCAGCGTCAGTTAAAGCCCAGCA730.24429422394752695No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCATTTACAGTCCAGTA710.23760123151060838No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAGTGTCAGTTACAGTCCAGCA700.2342547352921491No Hit
CCTGTTTGATACCCGCACTTTCGAGCCTCAGCGTCAGTTATGCCCCGGCA690.23090823907368987No Hit
CCCGTTTGCTACCCTAGCTTTCGTTCCTCAGCGTCAATAACGGCCCAGTG690.23090823907368987No Hit
CCTGTTTGATCCCCATGCTTTCGTGCCTCAGCGTCAGTATATGGCCAGAT650.21752225419985277No Hit
CCTGTTTGATCCCCATGCTTTCGAGCCTCAGCGTCAATATACAGCCAGAT650.21752225419985277No Hit
CCCATTTGCTACCCTGGCTTTCGTTCCTCAGTGTCAATTACGGCCCAGTA610.20413626932601564No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAGCGTCAGTACCAGTCCAGCA570.19075028445217856No Hit
CCCATTTGCTACCCCGGCTTTCGTTCCTCAGTGTCAATAACGGCCCAGTA560.18740378823371928No Hit
CCTGTTTGCTACCCATGCTTTCGAGCCTCAGCGTCAGTTGGTGCCCAGTA550.18405729201526No Hit
CCTGTTTGATCCCCATGCTTTCGTGCCTCAGCGTCAGTTGACAGCCAGAT490.16397831470450439No Hit
CCTGTTTGCTCCCCACGCTTTCGCCCCTCAGCGTCACTACCGGTCCAGGT470.15728532226758585No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAGCGTCCGATATAGATTTGTA460.15393882604912656No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTTCAGTCCAGAA440.147245833612208No Hit
CCTGTTCGATACCCACGCTTTCGTGCTTCAGCGTCAGTTATAGTATGGTA430.14389933739374874No Hit
CCTGTTCGCTCCCCTAGCTTTCGCGCCTCAGCGTCAGTGGCGGCCCAGAA410.1372063449568302No Hit
CCTGTTCGCTACCCACGCTTTCGTGCATCAGCGTCAGCTATAGATTAGCA400.13385984873837092No Hit
CCTGTTTGCTCCCCACACTTTCGTGCCTCAGTGTCAGTTTCAGTCCAGAA390.13051335251991167No Hit
CCTGTTCGCTACCCACGCTCTCGTGCATCAGCGTCAGTTACAGTCTGGTA390.13051335251991167No Hit
CCTGTTTGATACCCGCACTTTCGAGCATCAACGTCAGTTACGCTCCAGCA390.13051335251991167No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTCATCGTCCAGTA380.12716685630145239No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCACCGTCAGTTGTCGTCCAGTA360.12047386386453383No Hit
CCTGTTTGCTACCCATGCTTTCGTGCCTCAGCGTCAGTTAAAGCCCAGTA350.11712736764607455No Hit
CCTGTTTGCTCCCCATGCTTTCGTCCCTGAGTGTCAGTAATCGTCCAGAA310.10374138277223746No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTCGC203.0875162E-7213.000023
ATCCCCA2800.0213.000029
CGATACC700.0213.000027
TCGATAC700.0213.000026
TTCGATA700.0213.000025
TGATCCC2800.0213.000027
TGTTCGA700.0213.000023
CCCATTT203.0875162E-7213.000021
CATTTGC203.0875162E-7213.000023
CCGTTCG203.0875162E-7213.000022
CCCGTTC203.0875162E-7213.000021
CCATTTG203.0875162E-7213.000022
GATCCCC2800.0213.000028
TTGATCC2800.0213.000026
GTTCGAT700.0213.000024
TTTGATC2800.0213.000025
CCTATTT650.0213.01
CCTGTTC1250.0213.01
CTATTTG650.0213.02
TTCGCTC152.6299194E-5212.999985
Produced by FastQC (version 0.11.5)