FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005562465

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005562465
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences44330249
Sequences flagged as poor quality0
Sequence length32-101
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCTCTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTAC883960.19940334645988564No Hit
CCCCACTACCACAAATTATGCAGTCGAGTTTCCCACATTTGGGGAAATCG728840.1644114383386387No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCAGACG356650.049.9518472
ACGTTCG384800.046.382196
CGTTCGA404400.044.1692287
GGCAGAC424900.042.779341
AGACGTT420400.042.5795254
GACGTTC429450.041.8347635
GTTCGAA429500.041.785918
CAGACGT449600.040.455613
TTCGAAT465800.038.9857869
CTCGCTA123950.038.504961
AGATCGG144800.037.78298694-95
TGGAGTC930500.037.773662
GGAGTCT940350.037.473523
CGCTATG125100.037.2651183
CAGAACT1334300.036.88325594-95
TCGCTAT129900.036.688862
AGTCTTG999300.035.4560745
GAGTCTT1002950.035.139294
GTCGGTT41050.035.0641371
GTCTTGG1020100.035.0340586
Produced by FastQC (version 0.11.5)