FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005562493

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005562493
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences33366265
Sequences flagged as poor quality0
Sequence length32-126
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGGGAAGAAAGTTAGATTTACGCCGATGAATATGATAGTGAAATGGATT390290.11697143806776095No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGATCGG370400.0139.45667120
TAGATCG143850.065.623695120
CAGATCG245150.062.00484120
GAGATCG314900.059.45278120
AAGATCG165000.053.32747120
GGTGGTA694950.050.3048551
GATCCTC737650.044.442112120
GGTAGTA814150.043.4324874
TGGTAGT827250.042.5718273
GGGGAGT559150.042.4472271
AGGGACG980200.042.43785120
CCCTTCG179600.041.6730082
GTGGTAG852000.041.3351482
TTCGTCC188750.039.4634135
GTAGTAG893000.039.444015
CCTTCGT194400.039.021483
TAGTAGC915250.038.348396
AGTAGCA935000.038.0162357
CTTCGTC198900.037.5394444
CGTCCTG204750.037.2234737
Produced by FastQC (version 0.11.5)