FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005562494

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005562494
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences33366265
Sequences flagged as poor quality0
Sequence length32-126
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGATCGG377100.0143.77829120
CAGATCG386750.064.66256120
GGGCGAT58250.051.9714621
GAGATCG454350.046.86065120
GGCGATC66250.045.5129432
CGGTGGC304100.043.747341
TGGCGCG300900.041.2096254
GGCGCGT308100.040.2831155
GGTGGCG317600.039.7423862
GTGGCGC318400.039.3792273
CGATCTG83650.036.7557834
GCGATCT94550.032.5212063
GCGTGCC387600.031.8973668
GCGCGTG392000.031.804046
AAGATCG138500.031.529099120
GCGGTGG210500.030.436911
CGCGTGC414050.029.8099567
CCAGATC435000.029.283901120
CGTGCCT458900.027.4765139
ACAGATC353650.022.60265120
Produced by FastQC (version 0.11.5)