FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005562514

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005562514
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences36060801
Sequences flagged as poor quality0
Sequence length32-126
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGATCGG240800.0114.79043120
CAGATCG267300.052.883923120
GGGCGAT54650.043.6350671
GAGATCG294100.041.77194120
GGCGATC70450.032.998972
GGCGCGT43000.032.09815
GCGATCT90500.025.8857153
TAGATCG49200.024.483345120
CGATCTG102950.024.4362724
GCGCGTG59000.023.4683066
CGCGTGC64850.021.085667
TGGCGCG76250.017.8679284
GTGGCGC84800.016.4203623
CCAGATC418700.015.60414120
AGAGATC432950.014.887868120
GCGTGCC96000.014.744258
GACGACC423400.014.2984942
CTTGACG66950.014.2830941
GACCGGT585800.014.0449535
ACCGGTC590400.013.9400296
Produced by FastQC (version 0.11.5)