FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005562516

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005562516
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences36587862
Sequences flagged as poor quality0
Sequence length32-126
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGATCGG199600.0123.67449120
TAGATCG87300.055.86165120
CAGATCG158650.046.208435120
GAGATCG223300.045.385963120
GATCCTC873450.044.94819120
GTCGGTT62700.039.958461
CGATCCT733000.037.962975118-119
AAGATCG114700.037.39297120
ATCCTCC861150.034.55927120
GTCCGGT52050.032.2810671
AGCGATC866050.031.817614116-117
GTCCCGT93600.030.9833351
GGGGGGT252300.030.8808631
GCTCCGT312500.030.7296621
CCCCTCC1098800.030.6210821
CAAGCGA914900.030.583368114-115
GTCCGTT51200.030.3644661
CCCCCAT257950.029.2772371
CCCCCCT161850.028.2625271
GTCCGAT33400.027.9280361
Produced by FastQC (version 0.11.5)