FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005562525

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005562525
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences38254098
Sequences flagged as poor quality0
Sequence length32-101
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGTGGC478000.057.3917121
GGTGGCG658200.041.1640972
TGGCGCG800500.033.833824
GTGGCGC842950.032.119863
GGCGCGT884600.030.5686595
GCGCGTG924000.029.2571666
GGCGATC70650.028.3605982
AGATCGG92550.024.3609294-95
GCGACAT160050.023.0115017
TGCGACA160100.022.8284846
CGCGTGC1188600.022.7164557
GCGTGCC1229850.021.969768
GCGATCT91500.021.9493833
CGATCTG101500.020.9614851
GCTGCGA182000.020.1614344
CGACATC189150.019.5952668
CTGCGAC193750.018.9624615
ACGTGCG45500.018.560588
GGGCGAT115250.017.6456171
CCTGTAG1461950.017.58178512-13
Produced by FastQC (version 0.11.5)