FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005562542

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005562542
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences29818521
Sequences flagged as poor quality0
Sequence length32-126
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTGGTAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGAGA6157982.0651527283999096No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGATCGG236300.0133.1395120
TAGATCG95500.065.593285120
GGTGGTA1218500.063.5295681
GAGATCG206100.061.61821120
GTGGTAG1349300.057.7709122
CAGATCG165900.056.145462120
TGGTAGT1402850.055.59123
GGTAGTA1550000.050.4946944
GGGGAGT878500.050.190981
CCTTCGT249500.049.7574463
CCCTTCG249450.049.360472
AAGATCG94950.048.680878120
AGGGACG1956500.047.731434120
TCGGAAC110450.047.0958339
TTCGTCC270500.045.78425
CCGCTAT128550.045.6156162
GTAGTAG1713000.045.5434765
AGTAGCA1796750.043.8759167
TAGTAGC1801650.043.2726866
CGCTATT138050.043.1686333
Produced by FastQC (version 0.11.5)