FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005562543

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005562543
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences29818521
Sequences flagged as poor quality0
Sequence length32-126
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGATCGG240250.0136.60239120
CAGATCG264050.061.959026120
GGGCGAT50400.058.0271451
GGCGATC56850.050.8116952
GCGATCT65200.044.7622573
CGATCTG82450.035.973614
GAGATCG352350.035.381107120
TGGCGCG103650.034.673544
CGCAGGT95450.029.90856
GTGGCGC122750.029.5250633
TTCCGCA99050.028.740363
GGTGGCG130750.028.1306932
CTCGTTT634450.027.41111120
CCAGATC338800.026.67962120
AAGATCG97050.026.213535120
CTTCCGC110550.026.1297592
GCGTGCC146250.024.6823088
GGCGCGT149500.024.1179625
TCCGCAG124450.023.0642494
CGTGCCT172100.021.115029
Produced by FastQC (version 0.11.5)