FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005562547

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005562547
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences30966480
Sequences flagged as poor quality0
Sequence length32-126
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGATCGG260800.0143.74924120
CAGATCG271800.067.545235120
GGGCGAT45050.065.2926251
GGCGATC51100.058.262372
GCGATCT69650.042.401173
CGATCTG77950.038.4187284
TGGCGCG116500.037.968834
TTCCGCA118250.036.9057243
GAGATCG414050.036.11739120
CGCAGGT125650.035.207856
CTTCCGC129500.033.8848842
GTGGCGC133400.033.610693
CCAGATC312000.033.133717120
GGTGGCG137150.032.9974062
TCCGCAG135050.032.4437334
AAGATCG91000.030.932405120
GGCGCGT154800.028.5725575
GCGTGCC168050.026.415028
CTCGTTT954450.025.916283120
CGTGCCT184450.024.5857499
Produced by FastQC (version 0.11.5)