FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005562549

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005562549
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences36732820
Sequences flagged as poor quality0
Sequence length32-126
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGATCGG243950.0129.03925120
GAGATCG237400.053.78589120
TAGATCG112050.052.882484120
CAGATCG179950.052.43504120
GATCCTC628200.040.48532120
AAGATCG127550.040.081055120
CCCCCCT164250.040.0792121
GTCCGGT45700.039.51191
CGATCCT462150.037.082085118-119
GTCCCGT71300.036.05921
GGGGGGT261350.034.7285351
CCCCCAT256300.033.989761
GGGGGGG301850.033.277851
GGGGGCT362750.033.2457161
CCTGGCG179900.033.0719120
TCTATCG186400.032.1608965
AGCGATC543850.031.688408116-117
CCCCCTT365750.031.6325241
CCCCGAT73100.031.3269841
GTCCGTT64950.031.2994691
Produced by FastQC (version 0.11.5)