FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005562564

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005562564
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences82729437
Sequences flagged as poor quality0
Sequence length32-126
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGATCGG414750.0130.2559120
CACAGAC2377350.088.215911
ACAGACT2491600.084.063522
GGAACGT777750.080.609341
ACGTCTG972750.064.5691764
AACGTCT980600.064.009673
GAACGTC1016650.061.752112
CAGACTC3488550.059.9488953
CGTCTGA1049850.059.5846525
AGACTCA3601450.058.0690354
GACTCAG3636350.057.4998555
ACTCAGA3763900.055.7748156
CAGATCG433650.049.64145120
TAGATCG75100.046.149902120
GTCTGAG1445350.043.8249366
CTCAGAG4793650.043.6780787
TCAGAGA4946050.042.305858
CAGAGAG5252000.039.749419
TCTGAGG1455850.038.4039157
GAGATCG630700.035.679146120
Produced by FastQC (version 0.11.5)