FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005564511

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005564511
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences30451909
Sequences flagged as poor quality0
Sequence length100
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTGAGTCCAGGAGTTCTGGGCTGTAGTGCGCTATGCCGATCGGGTGTCC698740.22945687904163906No Hit
GTTCTGGGCTGTAGTGCGCTATGCCGATCGGGTGTCCGCACTAAGTTCGG691670.2271351855149705No Hit
GTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAGGAT659780.21666293564715436No Hit
CCCAAACCCACTCCACCTTACTACCAGACAACCTTAGCCAAACCATTTAC460470.15121219493989688No Hit
GTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGTGGGAGGAT458730.1506408021907592No Hit
CTGGGCTGTAGTGCGCTATGCCGATCGGGTGTCCGCACTAAGTTCGGCAT457770.15032555101882117No Hit
CACCAGGTTGCCTAAGGAGGGGTGAACCGGCCCAGGTCGGAAACGGAGCA418180.13732472404275214No Hit
GGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAGGATCG405080.13302285909234787No Hit
CTCGGCTCGTGCGTCGATGAAGAACGCAGCTAGCTGCGAGAATTAATGTG404130.13271089178678422No Hit
CCCAGCTACTCGGGAGGCTGAGGCTGGAGGATCGCTTGAGTCCAGGAGTT394680.12960763806301928No Hit
CTGGCTGCGACATCTGTCACCCCATTGATCGCCAGGGTTGATTCGGCTGA380290.12488215435032334No Hit
CTTGAGCCCAGGAGTTCTGGGCTGTAGTGCGCTATGCCGATCGGGTGTCC341980.11230166227017163No Hit
CGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAGGATCGCT319090.10478489213927443No Hit
CTAAGTTCGGCATCAATATGGTGACCTCCCGGGAGCGGGGGACCACCAGG305780.10041406599500871No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGCGCG266300.040.18842
GGCGCGT286950.037.4101753
GTGGCGC299400.036.043931
GCGCGTG331300.032.4446564
CGTGCGT136400.030.2917968
CGCGTGC370950.029.0905115
CGGCTCG150400.028.4688823
TCGTGCG151350.027.8553417
GCGACAT132750.027.155597
TCGGCTC166100.026.5541742
GGCTCGT156900.026.2109974
GGCGATC56250.025.8437441
GCGTGCC428450.025.482616
TGCGACA143650.024.7676686
CTCGTGC169700.024.6215156
CTCGGCT193650.024.0856481
CGTGCCT465000.023.4596027
GCTCGTG190400.022.1668645
GCGATCT64400.022.1236292
GATCGTT130550.021.48662694
Produced by FastQC (version 0.11.5)