FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005572662

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005572662
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences799395390
Sequences flagged as poor quality0
Sequence length18-102
%GC40

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAAATA972900.010.7008481
CGAATCG136250.010.32377051
CGAAAAA1503000.09.9285071
CGAAAAG1020600.09.6475561
CGATTAC551850.09.5714021
CGAAAGA1073800.09.1072031
CGGATTA499750.09.0566141
CGAAAAT1217700.08.9542981
CGAAAAC861450.08.8973341
CGAAACA923300.08.6847771
CGAAGTA547750.08.6123571
CGCTAAT484900.08.4952961
CGAAGAA1150100.08.3324531
CGAATTA611350.08.2485621
CGAAATT888950.08.2238191
CGAGAAA1205950.08.049711
CGAAATG1128250.07.80261141
CGAATAA850550.07.7907171
CGACGGA143400.07.64036041
CGAAGGA1018650.07.59473371
Produced by FastQC (version 0.11.5)