FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005572688

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005572688
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences799395390
Sequences flagged as poor quality0
Sequence length18-102
%GC40

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAAATA974850.010.5223631
CGAATCG140350.010.4994031
CGAAAAA1499350.09.8122451
CGAAAAG1020900.09.5556521
CGATTAC549950.09.3086541
CGAAAGA1063200.09.0899751
CGGATTA499400.09.0725161
CGAAAAT1218500.08.8894971
CGAAAAC855900.08.8264361
CGAAACA920150.08.7508811
CGAAGTA548000.08.6171351
CGCTAAT487550.08.3705911
CGAATTA610050.08.2896441
CGAAGAA1148750.08.2714191
CGAAATT883200.08.1906621
CGACGGA144800.08.1281711
CGAGAAA1202950.08.0538541
CGAAATG1126050.07.75410131
CGAAGGA1015050.07.71120361
CGAGAGA1151200.07.6636641
Produced by FastQC (version 0.11.5)