FastQCFastQC Report
Sun 11 Jun 2023
EGAF00005577085

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005577085
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21143002
Sequences flagged as poor quality0
Sequence length100
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCATTTCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCA552120.26113604870301765No Hit
ACTTTCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACC413130.19539798558407176No Hit
ACCCGAAAACCACGAGGAAGAGAGGTAGCGTTTTCTCCTGAGCGTGAAGC376770.17820080611069328No Hit
ACGTTTCTCTGAACGTGTAGAGCACCGAAAACCACGAGGAAGAGAGGTAG252080.11922621016637089No Hit
ACGTTCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACC234450.11088775378255179No Hit
ACCACGAGGAAGAGAGGTAGCGTTTTCTCCTGAGCGTGAAGCCGGCTTTT220550.10431347450092471No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)