FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005577261

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005577261
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences57027
Sequences flagged as poor quality0
Sequence length35-151
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GACTACAACCACGACCAATGATATGAAAAACCATCGTTGTATTTCAACTA940.16483420134322338No Hit
GTATAGGGGTTAGTCCTTGCTATATTATGCTTGGTTATAATTTTTCATCT780.13677731600820664No Hit
GCCCTACTCCACTCAAGCACTATAGTTGTAGCAGGAATCTTCTTACTCAT770.1350237606747681No Hit
CCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTGCAAGGAG710.1245024286741368No Hit
CCCCAGGGTTGGTCAATTTCGTGCCAGCCACCGCGGTCACACGATTAACC680.11924176267382118No Hit
GTATTAGAGGCACCGCCTGCCCAGTGACACATGTTTAACGGCCGCGGTAC660.11573465200694408No Hit
GTATAGGGCTGTGACTAGTATGTTGAGTCCTGTGAGTAGGAGAGTGATAT650.11398109667350553No Hit
CCTCTAGCATCACCAGTATTAGAGGCACCGCCTGCCCAGTGACACATGTT630.11047398600662844No Hit
CATTCATACAGGTCCCTATTTAAGGAACAAGTGATTATGCTACCTTTGCA620.1087204306731899No Hit
CTCCTAGCCTTACTACTAATAATCATTACATTTTGACTACCACAACTCAA610.10696687533975134No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN610.10696687533975134No Hit
GGCTAGGAGGGTGTTGATTATTAAAATTAAGGCGAAGTTTATTACTCTTT600.1052133200063128No Hit
GGCCTAGACTACGTACATAACCTAAACCTACTCCAATGCTAAAACTAATC580.10170620933943571No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)