FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005577268

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005577268
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13763
Sequences flagged as poor quality0
Sequence length35-151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN250.18164644336263897No Hit
GTACATGCTAAGACTTCACCAGTCAAAGCGAACTACCATACTCAATTGAT160.11625372375208894No Hit
CATTTACACCAACCACCCAACTATCTATAAACCTAGCCATGGCCATCCCC140.10172200828307781No Hit
ATATTATGCTTGGTTATAATTTTTCATCTTTCCCTTGCGGTACTATATCT140.10172200828307781No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGATAT200.007380805141.92741145
CAATGCA350.005723135554.8540234
GCAGCTC350.005723135554.8540238
ATGCAGC350.005723135554.8540236
TGCAGCT350.005723135554.8540237
AATGCAG400.00970356747.9972765
CTACCAT200.004032355731.483007105-109
Produced by FastQC (version 0.11.5)