FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005577269

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005577269
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13763
Sequences flagged as poor quality0
Sequence length35-151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN250.18164644336263897No Hit
GTATAGGGGTTAGTCCTTGCTATATTATGCTTGGTTATAATTTTTCATCT200.14531715469011117No Hit
GCCTATATACCGCCATCTTCAGCAAACCCTGATGAAGGCTACAAAGTAAG190.1380512969556056No Hit
CATTTATATAGAGGAGACAAGTCGTAACATGGTAAGTGTACTGGAAAGTG150.10898786601758338No Hit
GTATATAGGCTGAGCAAGAGGTGGTGAGGTTGATCGGGGTTTATCGATTA140.10172200828307781No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACACAAT350.005775548554.727796
TGGGGTT400.00979229647.8868185
CTAAACC400.00979229647.8868189
Produced by FastQC (version 0.11.5)