FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005577272

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005577272
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12266
Sequences flagged as poor quality0
Sequence length35-151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN260.21196804174139897No Hit
CATTGGGACAGACCTAGTTCAATGAATCTGAGGAGGCTACTCAGTAGACA220.17935757378118375No Hit
ATATAAATGCGTAGGGGTTTTAGTTAAATGTCCTTTGAAGTATATTTGAG180.14674710582096853No Hit
AGCATGTACTGCTCGGAGGTTGGGTTCTGCTCCGAGGTCGCCCCAACCGA170.1385944888309147No Hit
AAGCTATACTAACCCCAGGGTTGGTCAATTTCGTGCCAGCCACCGCGGTC150.12228925485080712No Hit
GTATAGTACGGATGCTACTTGTCCAATGATGGTAAAAGGGTAGCTTACTG150.12228925485080712No Hit
GTATAGGGGTTAGTCCTTGCTATATTATGCTTGGTTATAATTTTTCATCT140.11413663786075331No Hit
ACTATATCTATTGCGCCAGGTTTCAATTTCTATCGCCTATACTTTATTTG140.11413663786075331No Hit
GTACATGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTCAATTGAT140.11413663786075331No Hit
CCCCAGGGTTGGTCAATTTCGTGCCAGCCACCGCGGTCACACGATTAACC130.10598402087069948No Hit
ATCCTAGGCCTACCCGCCGCAGTACTGATCATTCTATTTCCCCCTCTATT130.10598402087069948No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGAGTT109.365801E-4282.73914145
TAAACCA150.0031528054188.49275145
GTGTATA100.009128991132.714282
CCTAACT100.009128991132.714283
CTGTTAA100.009128991132.714289
TGTATAT100.009128991132.714283
Produced by FastQC (version 0.11.5)